The object discrimination test allows the testing of different memory retention periods. However, few behavioral endpoints have been measured in fish species such that retention is often assessed using a single parameter (time spent in object area). Here, we aimed to explore the object discrimination test in zebrafish by assessing their behavioral performance after 1 or 24 h retention interval periods. To characterize putative interaction-like behaviors, fish were tested in the absence or presence of scopolamine (1 h before test session). Zebrafish were habituated for 3 consecutive days in the experimental tank, and training session was performed for 10 min using two identical nonpreferred objects (black cube or sphere). After the retention intervals, a familiar object was replaced by a novel object (test session, 10 min). Fish were also exposed to the novel tank diving test to assess locomotion and anxiety-like behaviors. At 1 h retention interval, animals performed more circular-like investigation near the familiar object, whereas 24 h after training session, a prominent rapid investigation was observed when animals explore the nonfamiliar object. Because scopolamine abolished these phenotypes, as well as the increased time spent in the novel object area during the test without changing locomotion and anxiety-related parameters, the behavioral responses described here may predictively reflect interaction-like behaviors involved in object discrimination memory in zebrafish models.

中文翻译：

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探索斑马鱼中的对象歧视：在不同的保留时间间隔的行为表现和S胺引起的认知缺陷。

对象辨别测试允许测试不同的内存保留期。但是，在鱼类中几乎没有行为终点，因此经常使用单个参数（在目标区域花费的时间）来评估保留时间。在这里，我们旨在通过评估1或24 h保留间隔期后它们的行为表现来探索斑马鱼中的物体识别测试。为了表征推定的类似相互作用的行为，在不存在或存在东pol碱的情况下（测试前1小时）对鱼进行了测试。斑马鱼在实验缸中连续培养了3天，并使用两个相同的非优选对象（黑方块或球形）进行了10分钟的训练。在保留间隔之后，将熟悉的对象替换为新对象（测试会话，10分钟）。鱼还接受了新型的坦克潜水测试，以评估运动和焦虑样行为。在1 h的保留时间间隔内，动物在熟悉的物体附近进行了更多的圆形检查，而训练后24 h，当动物探索不熟悉的物体时，观察到了显着的快速检查。因为东pol碱废除了这些表型，并且在测试过程中花费在新颖的对象区域上的时间增加而没有改变运动和焦虑相关参数，所以此处描述的行为反应可以预测性地反映斑马鱼模型中对象识别记忆中涉及的类似交互行为。 。而训练后24小时，当动物探索不熟悉的物体时，观察到了显着的快速调查。因为东pol碱废除了这些表型，并且在测试过程中花费在新颖的对象区域上的时间增加而没有改变运动和焦虑相关参数，所以此处描述的行为反应可以预测性地反映斑马鱼模型中对象识别记忆中涉及的类似交互行为。 。而训练后24小时，当动物探索不熟悉的物体时，观察到了显着的快速调查。因为东pol碱废除了这些表型，并且在测试过程中花费在新颖的对象区域上的时间增加而没有改变运动和焦虑相关参数，所以此处描述的行为反应可以预测性地反映斑马鱼模型中对象识别记忆中涉及的类似交互行为。 。