This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography–mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high‐performance liquid chromatography (HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer (MDA‐MB 231) was treated with a purified vinblastine which was intracellulary produced by N. sphaerica. The purified vinblastine from extracted leaf of C. roseus was used as a standard comparison. A positive result with a value of half maximal inhibitory concentration (IC₅₀) of > 32 μg ml⁻¹ was observed compared with standard (IC₅₀) of 350 μg ml⁻¹ only. It showed that a vinblastine produced by N. sphaerica has a high cytotoxicity activity even though the concentration of vinblastine produced by this endophytic fungus was only 0.868 μg ml⁻¹.

中文翻译：

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首次报道从长春花植物中分离出具有抗癌特性的新型球形黑孢子菌。


本文报道了从长春花中分离出的新型球形黑孢菌产生的长春花生物碱。通过液相色谱-质谱法（LCMS），只有该真菌的粗菌丝体提取物对长春花碱的测定呈阳性。然后使用高效液相色谱 (HPLC) 纯化这种长春花生物碱，并使用 MTT 测定法测试细胞毒性活性。用球形长春花胞内产生的纯化长春花碱治疗乳腺癌细胞系癌症 (MDA-MB 231)。从提取的玫瑰花叶中纯化的长春花碱用作标准比较。与仅350μgml ^-1的标准品(IC ₅₀ )相比，观察到半数最大抑制浓度(IC ₅₀ )值>32μgml ^-1的阳性结果。结果表明，尽管该内生真菌产生的长春碱浓度仅为0.868μg ml ^-1 ，但由球形N. sphaerica产生的长春碱具有高细胞毒活性。