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UPLC-MS/MS Determination of Twelve Ginsenosides in Shenfu Tang and Dushen Tang.
International Journal of Analytical Chemistry ( IF 1.5 ) Pub Date : 2019-07-11 , DOI: 10.1155/2019/6217125
Dawai Yang 1 , Xiaofang Yang 2 , Han Yan 2 , Bin Fan 2 , Jingang Dai 2 , Jun Song 2 , Yan Lei 2 , Na Guo 2
Affiliation  

Shenfu Tang and Dushen Tang (one of the composite medicines for Shenfu Tang) are widely used Traditional Chinese herbal formulations and ginsenosides are their main bioactive components. However, there are rare studies about simultaneous analysis of ginsenosides in Shenfu Tang and Dushen Tang. In order to identify ginsenosides in Shenfu Tang and Dushen Tang and to explore law of compatibility of medicines in the decoction, a method for simultaneous determination of twelve ginsenosides in Shenfu Tang and Dushen Tang was developed by ultraresolution liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). The method showed satisfactory linearity (r > 0.9915), repeatability (RSD < 9.58%), intra- and interday precisions (RSD<11.90%), and high yields of recovery (92.26-113.20%) for twelve major constituents, namely, ginsenosides-Rb1, Rb2, Rb3, Rc, Rd, Rg1, Re, Rf, Rg2, Rg3, Rh1, and F2. Furthermore, the concentration of twelve ginsenosides in Dushen Tang and Shenfu Tang was also simultaneously analyzed. Most of ginsenosides except Rg1 and Rb1 showed higher contents in Shenfu Tang compared to Dushen Tang. The compatibility of the formula had the effect of promoting or inhibiting the dissolution of some major components. The present research provided a reliable evidence for the illustration of chemical basis and compatibility regularity of Shenfu Tang. This study demonstrated the utility of the developed method for assessment of the quantity of the major constituents in Dushen Tang and Shenfu Tang.

中文翻译:

UPLC-MS / MS法测定神甫汤和独神汤中的十二种人参皂苷。

参附汤和独参汤(参附汤的一种复合药物)被广泛使用中草药制剂,人参皂甙是它们的主要生物活性成分。然而,关于神甫汤和独肾汤中人参皂苷的同时分析研究很少。为了鉴别参附汤和独参汤中的人参皂苷,并探讨该汤中药物的相容性规律,建立了超分辨液相色谱-串联质谱法同时测定参附汤和独参汤中十二种人参皂苷的方法。 -MS / MS)。该方法显示出令人满意的线性(r> 0.9915),重复性(RSD <9.58%),日内和日间精度(RSD <11.90%)和十二种主要成分的高回收率(92.26-113.20%),即 人参皂甙Rb1,Rb2,Rb3,Rc,Rd,Rg1,Re,Rf,Rg2,Rg3,Rh1和F2。此外,还同时分析了独参汤和神府汤中十二种人参皂甙的浓度。与独神汤相比,除Rg1和Rb1外,大多数人参皂苷在神府汤中的含量较高。配方的相容性具有促进或抑制某些主要成分溶解的作用。本研究为说明神府汤的化学基础和相容性规律提供了可靠的证据。这项研究证明了所开发的方法可用于评估独肾汤和神甫汤中主要成分的数量。同时对独参汤和神府汤中十二种人参皂甙的含量进行了分析。与独神汤相比,除Rg1和Rb1外,大多数人参皂苷在神府汤中的含量较高。配方的相容性具有促进或抑制某些主要成分溶解的作用。本研究为说明神府汤的化学基础和相容性规律提供了可靠的证据。这项研究证明了所开发的方法可用于评估独肾汤和神甫汤中主要成分的数量。同时对独参汤和神府汤中十二种人参皂甙的含量进行了分析。与独神汤相比,除Rg1和Rb1外,大多数人参皂苷在神府汤中的含量较高。配方的相容性具有促进或抑制某些主要成分溶解的作用。本研究为说明神府汤的化学基础和相容性规律提供了可靠的证据。这项研究证明了所开发的方法可用于评估独肾汤和神甫汤中主要成分的数量。本研究为说明神府汤的化学基础和相容性规律提供了可靠的证据。这项研究证明了所开发的方法可用于评估独肾汤和神甫汤中主要成分的数量。本研究为说明神府汤的化学基础和相容性规律提供了可靠的证据。这项研究证明了所开发的方法可用于评估独肾汤和神甫汤中主要成分的数量。
更新日期:2019-07-11
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