The aim of this study was to assess the distribution of runs of homozygosity (ROH) and autozygosity islands in the composite Montana Tropical® beef cattle to explore hotspot regions which could better characterize the different biological types within the composite breed. Montana animals (n = 1,436) were genotyped with the GGP-LD BeadChip (~30,000 markers). ROH was identified in every individual using the plink v1.90 software. Medium and long ROH prevailed in the genome, which accounted for approximately 74% of all ROH detected. On an average, 2.0% of the genome was within ROH, agreeing with the pedigree-based inbreeding coefficient. The Montana cattle with a higher proportion of productive breed types showed the highest number of autozygosity islands (n = 17), followed by those with a higher proportion of breeds adapted to tropical environments (n = 15). Enriched terms (p < .05) associated with the immune and inflammatory response, homeostasis, reproduction, mineral absorption, and lipid metabolism were described within the autozygosity islands. In this regard, over-represented GO terms and KEGG pathways described in this population may play a key role in providing information to explore the genetic and biological mechanisms together with the genomic regions underlying each biological type that favoured their optimal performance ability in tropical and subtropical regions.

中文翻译：

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对复合蒙大拿热带® 肉牛纯合子运行的全基因组扫描

本研究的目的是评估复合蒙大拿热带® 肉牛中纯合子 (ROH) 和自合子岛的分布，以探索可以更好地表征复合品种内不同生物类型的热点区域。蒙大拿州动物 (n = 1,436) 使用 GGP-LD BeadChip（~30,000 个标记）进行基因分型。使用 plink v1.90 软件在每个人中识别出 ROH。基因组中以中等和长 ROH 为主，约占检测到的所有 ROH 的 74%。平均而言，2.0% 的基因组在 ROH 内，与基于谱系的近亲繁殖系数一致。生产品种比例较高的蒙大拿牛显示出最多的自合岛（n = 17），其次是适应热带环境的品种比例较高的品种（n = 15）。在自接合岛中描述了与免疫和炎症反应、体内平衡、繁殖、矿物质吸收和脂质代谢相关的丰富术语 (p < .05)。在这方面，该群体中描述的过度表达的 GO 术语和 KEGG 通路可能在提供信息以探索遗传和生物学机制以及每种生物类型背后的基因组区域方面发挥关键作用，这些区域有利于它们在热带和亚热带的最佳表现能力。地区。