Labeling RNA is a recurring problem to make RNA compatible with state-of-the-art methodology and comes in many flavors. Considering only cellular applications, the spectrum still ranges from site-specific labeling of individual transcripts, for example, for live-cell imaging of mRNA trafficking, to metabolic labeling in combination with next generation sequencing to capture dynamic aspects of RNA metabolism on a transcriptome-wide scale. Combining the specificity of RNA-modifying enzymes with non-natural substrates has emerged as a valuable strategy to modify RNA site- or sequence-specifically with functional groups suitable for subsequent bioorthogonal reactions and thus label RNA with reporter moieties such as affinity or fluorescent tags. In this review article, we will cover chemo-enzymatic approaches (a) for in vitro labeling of RNA for application in cells, (b) for treatment of total RNA, and (c) for metabolic labeling of RNA. This article is categorized under: RNA Processing < RNA Editing and Modification RNA Methods < RNA Analyses in vitro and In Silico RNA Methods < RNA Analyses in Cells.

中文翻译：

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化学酶法处理RNA以促进分析。

标记RNA是使RNA与最新方法兼容的一个反复出现的问题，并且有许多种口味。仅考虑细胞应用，光谱范围仍然从单个转录本的位点特异性标记（例如，用于mRNA交易的活细胞成像）到与下一代测序结合以在转录组上捕获RNA代谢动态方面的代谢标记，大规模。将RNA修饰酶的特异性与非天然底物相结合已成为一种有价值的策略，可利用适合于后续生物正交反应的官能团对RNA进行位点或序列特异性修饰，从而用报道分子（如亲和力或荧光标签）标记RNA。在这篇评论文章中，我们将介绍化学酶法（a）体外标记RNA在细胞中的应用，（b）处理总RNA，以及（c）RNA的代谢标记。本文归类为：RNA处理<RNA编辑和修饰RNA方法<体外RNA分析和计算机模拟RNA方法<细胞中RNA分析。