Nowadays, Mesenchymal stem cells (MSCs) have become one of the most attractive tools for treating tumors, due to their specific characteristics, the most prominent of which are tropism toward tumor. These cells will exert their effects through their secretion. In this study, our aim was to evaluate the anti-cancer effect of umbilical cord-derived mesenchymal cells (UCMSC) secretome, on MCF-7 tumor cells. MSCs were extracted from the umbilical cord of mothers, having normal delivery or cesarean section. After culture, the supernatants of these cells were collected and freeze-dried. The cytotoxic effect of freeze-dried secretome was examined at different concentrations on MCF-7 and the optimum concentrations (IC50) were calculated, using MTT assay. These results were confirmed by BrdU assay. The effect of induction of apoptosis of the MSC secretome on MCF-7 was determined, using annexin V/PI method by flow cytometry. The results of our study indicate that the isolation and growth time of UCMSCs of mothers who were naturally delivered was lower than those who received cesarean section. Co-culture studies showed that MSCs had cytotoxic effects on MCF-7 cells. The MSC secretome also showed cytotoxic effects on the MCF-7 cell line, this effect was mediated by induction of apoptosis, which was dose-dependent with an IC50 of 10 mg/mL.

中文翻译：

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脐带间充质干细胞分泌物组对乳腺癌细胞系的抗癌作用。

如今，间充质干细胞（MSCs）由于其独特的特性已成为治疗肿瘤的最有吸引力的工具之一，其中最突出的是对肿瘤的向性。这些细胞将通过分泌发挥作用。在这项研究中，我们的目的是评估脐带来源的间充质细胞（UCMSC）分泌组对MCF-7肿瘤细胞的抗癌作用。从正常分娩或剖宫产的母亲的脐带中提取MSC。培养后，收集这些细胞的上清液并冷冻干燥。用MTT法检测了不同浓度的冻干分泌物组对MCF-7的细胞毒性作用，并计算了最佳浓度（IC50）。这些结果通过BrdU测定法得到证实。用膜联蛋白V / PI法通过流式细胞术确定了MSC分泌组凋亡诱导MCF-7的作用。我们的研究结果表明，自然分娩的母亲的UCMSCs的分离和生长时间要短于剖宫产的母亲。共培养研究表明，MSC对MCF-7细胞具有细胞毒性作用。MSC分泌基因组还对MCF-7细胞系显示出细胞毒性作用，这种作用是通过诱导凋亡来介导的，凋亡的作用与剂量有关，IC50为10 mg / mL。共培养研究表明，MSC对MCF-7细胞具有细胞毒性作用。MSC分泌组还对MCF-7细胞系显示出细胞毒性作用，这种作用是通过诱导细胞凋亡来介导的，凋亡的作用与剂量有关，IC50为10 mg / mL。共培养研究表明，MSC对MCF-7细胞具有细胞毒性作用。MSC分泌组还对MCF-7细胞系显示出细胞毒性作用，这种作用是通过诱导细胞凋亡来介导的，凋亡的作用与剂量有关，IC50为10 mg / mL。