This study is the first to investigate and confirm the effectiveness of single-cell Raman spectroscopy (SCRM), in its ability to discriminate between dental pulp stem cells (DPSCs) with contrasting proliferative and differentiation capabilities. The findings show that SCRM can rapidly and noninvasively distinguish and identify DPSC subpopulations in vitro with superior proliferative and multipotency properties, versus lesser quality DPSCs, thereby overcoming the significant heterogeneity issues surrounding DPSC ex vivo expansion and differentiation capabilities. Such findings support further SCRM assessment for the selective screening/isolation of superior quality DPSCs from whole dental pulp tissues, for more effective in vitro evaluation and therapy development.

中文翻译：

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通过单细胞拉曼光谱法鉴别牙髓干细胞再生异质性。

这项研究是第一个研究和证实单细胞拉曼光谱法（SCRM）的有效性，该方法具有区别牙髓干细胞（DPSC）的增殖能力和分化能力的能力。研究结果表明，相对于质量较差的DPSC，SCRM可以在体外快速，无创地区分和鉴定具有优越的增殖和多能性的DPSC亚群，从而克服了围绕DPSC离体扩增和分化能力的重大异质性问题。这些发现支持进一步的SCRM评估，以便从整个牙髓组织中选择性筛选/分离出高质量的DPSC，从而更有效地进行体外评估和治疗开发。