Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoospermic samples. Forty normozoospermic specimens were included in this prospective study. Each sample was divided into ten groups. In groups one to five, 0 (as control group) 5, 10, 20 and 30 µg/ml C. siliqua were added to freezing medium and in groups six to ten, similar concentration of C. siliqua were added to thawing medium for 30 min incubation. Sperm concentration, progressive motility, normal morphology, viability, aniline blue (AB), toluidine blue (TB) and sperm chromatin dispersion (SCD) staining tests were evaluated before vitrification and after thawing. The results showed that 10 and 20 µg/ml supplementation of C. siliqua in freezing/thawing media significantly increased progressive motility, normal morphology and viability of sperm (p < 0.05) as well as decreased AB, TB and SCD (p < 0.05). Also, 20 µg/ml had significantly higher improvement compared to 10 µg/ml C. siliqua (p < 0.05). The present study showed that C. siliqua supplemented freezing/thawing media can improve sperm quality of normozoospermic samples after freezing/thawing.

中文翻译：

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硅角藜添加精子冻融介质是否能改善冷冻保存对正常精子标本中精子参数和染色质质量的有害影响？

人类精子库是辅助生殖技术中心的重要程序。它会导致精子受损。这项研究的目的是调查在冷冻/解冻介质中添加的角柏藜（C. siliqua）补充剂对正常精子样本中融化后精子参数和精子染色质质量的有益影响。这项前瞻性研究包括40个正常精子标本。每个样品分为十组。在第1至5组中，将0（作为对照组）分别向冷冻培养基中添加5、10、20和30 µg / ml的C. siliqua；在第6至10组中，将类似浓度的C. siliqua将其加入解冻培养基中孵育30分钟。在玻璃化之前和融化之后，评估精子浓度，进行性运动，正常形态，生存力，苯胺蓝（AB），甲苯胺蓝（TB）和精子染色质分散液（SCD）染色测试。结果表明，在冷冻/解冻培养基中补充硅纤梭菌10和20 µg / ml显着提高了精子的进行性运动能力，正常形态和生存力（p  <0.05）以及降低了AB，TB和SCD（p  <0.05） 。此外，与10 µg / ml的硅酸棒菌相比，20 µg / ml的改善显着更高（p  <0.05）。本研究表明，C。siliqua 补充的冷冻/解冻介质可以提高冷冻/解冻后正常精子样品的精子质量。