Regeneration of periodontal tissues is affected by the biological and morphological characteristics of the membrane surface. The current study evaluated the adhesion of human gingival fibroblasts (HGF) and MG-63 osteoblast-like cells to Membranes, with and without activated PRP. The line of human gingival fibroblast cells and MG-63 osteoblast-like cells were first prepared and cultured on three types of membranes, including Jason, CenoMembrane and TXT-200 in three groups (FBS 10%, FBS 0.5% and activated PRP). Cell viability was investigated by MTT assay and electron microscopy (SEM) was used to evaluate the cell morphology and adhesion on these membranes after 24 and 72 h. Two-way ANOVA was carried out at the significance level of 0.05. The highest adhesion in the 10% FBS group for HGF and The MG-63 osteoblast-like cells was observed to the Jason membrane during 24 h and 72 h (p < 0.05). However, there were no significant differences among the three membranes in PRP and FBS groups for HGF during 24 h and for MG-63 cells during 72 h (p > 0.05). Activated PRP had a positive effect on the viability and adhesion of both human gingival fibroblasts and osteoblast-like cells as compared to the FBS 0.5% group, but these effects were not as 10% FBS group. The results also showed that Jason membrane had the highest amount of cell viability and adhesion.

中文翻译：

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评估人牙龈成纤维细胞和MG-63成骨细胞样细胞对活化的PRP涂层膜的粘附力。

牙周组织的再生受膜表面的生物学和形态特征影响。目前的研究评估了在有和没有活化PRP的情况下，人牙龈成纤维细胞（HGF）和MG-63成骨细胞样细胞对膜的粘附性。首先制备人牙龈成纤维细胞和MG-63成骨细胞样细胞系，并在三种类型的膜上进行培养，包括Jason，CenoMembrane和TXT-200，分为三组（FBS为10％，FBS为0.5％和活化的PRP）。通过MTT测定研究细胞活力，并在24和72小时后使用电子显微镜（SEM）评估这些膜的细胞形态和粘附。在0.05的显着性水平上进行了两次方差分析。p  ＜0.05）。但是，PRP和FBS组的三层膜在24 h时的HGF和72 h时的MG-63细胞之间没有显着差异（p  > 0.05）。与FBS 0.5％组相比，活化的PRP对人牙龈成纤维细胞和成骨细胞样细胞的存活率和粘附均具有积极的作用，但对于10％FBS组则没有作用。结果还表明，Jason膜具有最高的细胞活力和粘附力。