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Magnetically Promoted Rapid Immunofluorescence Staining for Frozen Tissue Sections.
Journal of Histochemistry & Cytochemistry ( IF 1.9 ) Pub Date : 2019-04-08 , DOI: 10.1369/0022155419841023
Tatsuya Onishi 1, 2 , Sachiko Matsuda 1 , Yuki Nakamura 1 , Junko Kuramoto 3 , Akinori Tsuruma 4 , Satoshi Sakamoto 4 , Shunichi Suzuki 5 , Daiichiro Fuchimoto 5 , Akira Onishi 6 , Shinichi Chikaki 7 , Miki Kaneko 7 , Akihiro Kuwahata 7 , Masaki Sekino 7 , Hiroshi Yasuno 8 , Naohiro Hanyu 8 , Tomoko Kurita 9 , Hiroyuki Takei 9 , Takashi Sakatani 10 , Kanae Taruno 11 , Seigo Nakamura 11 , Tetsu Hayashida 1 , Hiromitsu Jinno 12 , Moriaki Kusakabe 13, 14 , Hiroshi Handa 15 , Kaori Kameyama 16 , Yuko Kitagawa 1
Affiliation  

Current immunohistochemistry methods for diagnosing abnormal cells, such as cancer cells, require multiple steps and can be relatively slow compared with intraoperative frozen hematoxylin and eosin staining, and are therefore rarely used for intraoperative examination. Thus, there is a need for novel rapid detection methods. We previously demonstrated that functionalized fluorescent ferrite beads (FF beads) magnetically promoted rapid immunoreactions. The aim of this study was to improve the magnetically promoted rapid immunoreaction method using antibody-coated FF beads and a magnet subjected to a magnetic field. Using frozen sections of xenograft samples of A431 human epidermoid cancer cells that express high levels of epidermal growth factor receptor (EGFR) and anti-EGFR antibody-coated FF beads, we reduced the magnetically promoted immunohistochemistry procedure to a 1-min reaction and 1-min wash. We also determined the optimum magnetic force for the antibody reaction (from 7.79 × 10-15 N to 3.35 × 10-15 N) and washing (4.78 × 10-16 N), which are important steps in this technique. Furthermore, we stained paraffin-embedded tissue arrays and frozen sections of metastatic breast cancer lymph nodes with anti-pan-cytokeratin antibody-coated FF beads to validate the utility of this system in clinical specimens. Under optimal conditions, this ultra-rapid immunostaining method may provide an ancillary method for pathological diagnosis during surgery. (J Histochem Cytochem 58:XXX-XXX, 2010).

中文翻译:

磁性促进的冷冻组织切片的快速免疫荧光染色。

当前用于诊断异常细胞(例如癌细胞)的免疫组织化学方法需要多个步骤,并且与术中冷冻的苏木精和曙红染色相比可能相对较慢,因此很少用于术中检查。因此,需要新颖的快速检测方法。我们以前证明功能化的荧光铁氧体磁珠(FF珠)磁性促进快速免疫反应。这项研究的目的是改善使用抗体包被的FF磁珠和受到磁场的磁体的磁性促进的快速免疫反应方法。使用表达高水平表皮生长因子受体(EGFR)和抗EGFR抗体包裹的FF珠粒的A431人表皮样癌细胞的异种移植样品的冷冻切片,我们将磁性促进的免疫组织化学程序减少为1分钟的反应和1分钟的清洗。我们还确定了抗体反应的最佳磁力(从7.79×10-15 N到3.35×10-15 N)和洗涤(4.78×10-16 N),这是该技术的重要步骤。此外,我们用抗泛细胞角蛋白抗体包被的FF珠对转移性乳腺癌淋巴结的石蜡包埋的组织阵列和冰冻切片进行染色,以验证该系统在临床标本中的实用性。在最佳条件下,这种超快速免疫染色方法可为手术过程中的病理诊断提供辅助方法。(J Histochem Cytochem 58:XXX-XXX,2010)。35×10-15 N)和洗涤(4.78×10-16 N),这是该技术的重要步骤。此外,我们用抗泛细胞角蛋白抗体包被的FF珠对转移性乳腺癌淋巴结的石蜡包埋的组织阵列和冰冻切片进行染色,以验证该系统在临床标本中的实用性。在最佳条件下,这种超快速免疫染色方法可为手术过程中的病理诊断提供辅助方法。(J Histochem Cytochem 58:XXX-XXX,2010)。35×10-15 N)和洗涤(4.78×10-16 N),这是该技术的重要步骤。此外,我们用抗泛细胞角蛋白抗体包被的FF珠对转移性乳腺癌淋巴结的石蜡包埋的组织阵列和冰冻切片进行染色,以验证该系统在临床标本中的实用性。在最佳条件下,这种超快速免疫染色方法可为手术过程中的病理诊断提供辅助方法。(J Histochem Cytochem 58:XXX-XXX,2010)。这种超快速的免疫染色方法可以为手术过程中的病理诊断提供辅助方法。(J Histochem Cytochem 58:XXX-XXX,2010)。这种超快速的免疫染色方法可以为手术过程中的病理诊断提供辅助方法。(J Histochem Cytochem 58:XXX-XXX,2010)。
更新日期:2019-11-01
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