The present study evaluates the effects of leukocyte-platelet-rich fibrin (L-PRF) combined with open flap debridement (OFD) on clinical parameters and growth factors levels (GFL) in chronic periodontitis (CP) patients. This trial was registered at clinicaltrials.gov as NCT02594605. 16 patients (32 sites) with chronic periodontitis who had at least two areas of horizontal bone loss, were treated with OFD alone or L-PRF with OFD (OFD + L-PRF). GFL in gingival crevicular fluid (GCF) were analyzed at baseline, 1 week, 2 weeks and 4 weeks after operation. Probing depth (PD) and clinical attachment level (CAL) were measured at baseline and 6 months postoperatively. PD reduction and CAL gain were significantly higher in the OFD + L-PRF sites than in OFD sites. OFD + L-PRF group showed significantly increased bone morphogenetic protein-2 and insulin-like growth factor-1 at 2 weeks compared with baseline. L-PRF combined with OFD significantly increases GFL and thus, it enhances the periodontal healing on CP patients.

本研究评估了白细胞-血小板丰富的纤维蛋白（L-PRF）联合开放皮瓣清创术（OFD）对慢性牙周炎（CP）患者临床参数和生长因子水平（GFL）的影响。该试验已在Clinicaltrials.gov上注册为NCT02594605。至少有两个水平骨丢失区域的16例慢性牙周炎患者（32个部位）接受了单独的OFD或L-PRF和OFD治疗（OFD + L-PRF）。在基线，术后1周，2周和4周时分析牙龈沟液（GCF）中的GFL。在基线和术后6个月测量探查深度（PD）和临床依从水平（CAL）。在OFD + L-PRF部位，PD降低和CAL增益显着高于在OFD部位。与基线相比，OFD + L-PRF组在第2周显示骨形态发生蛋白2和胰岛素样生长因子1显着增加。L-PRF与OFD联合可显着增加GFL，从而增强CP患者的牙周愈合。