Domestic Pigeon (Columba livia) is one of few domesticated birds with an important economic value. In this study, a comprehensive investigation on the morphology and cytochemical patterns of peripheral blood cells in domestic pigeons were conducted by using wright's and various cytochemical staining techniques including periodic acid-Schiff(PAS), sudan black B(SBB), peroxidase(POX), alkaline phosphatase(ALP), acid phosphatase(ACP), chloroacetic acid AS-D naphthol esterase(AS-D) and α-naphthol acetate esterase(α-NAE) staining. Besides erythrocytes and thrombocytes, five types of leukocytes were identified: heterophils, eosinophils, basophils, lymphocytes and monocytes. Lymphocytes were the most abundant leukocytes, followed by heterophils, eosinophils, monocytes; basophils were the fewest. Erythrocytes and thrombocytes were positive for PAS, and negative for all the other cytochemical staining. Heterophils and eosinophils exhibited positive to all cytochemical staining except for α-NAE. Basophils exhibited strongly positive for POX and AS-D, positive for PAS and ALP, while negative for SBB, ACP and α-NAE staining. Monocytes exhibited positive for PAS and α-NAE, and weakly positive for ACP, while negative for SBB, POX, ALP and AS-D staining. Lymphocytes showed positive for PAS and ACP, weakly positive for AS-D, while negative for SBB, POX, ALP and α-NAE staining. Our results add up knowledge about the domestic pigeon blood cells.

中文翻译：

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家鸽（Columba livia）外周血细胞的形态和细胞化学模式。

家鸽（Columba livia）是为数不多的具有重要经济价值的家禽之一。在这项研究中，通过使用赖特氏和包括高碘酸希夫（PAS），苏丹黑B（SBB），过氧化物酶（POX）在内的各种细胞化学染色技术，对家鸽外周血细胞的形态和细胞化学模式进行了全面研究。 ，碱性磷酸酶（ALP），酸性磷酸酶（ACP），氯乙酸AS-D萘酚酯酶（AS-D）和α-萘乙酸酯酯酶（α-NAE）染色。除红细胞和血小板外，还鉴定出五种白细胞：嗜异性粒细胞，嗜酸性粒细胞，嗜碱性粒细胞，淋巴细胞和单核细胞。淋巴细胞是最丰富的白细胞，其次是嗜异性粒细胞，嗜酸性粒细胞，单核细胞。嗜碱性粒细胞最少。PAS的红细胞和血小板细胞阳性，而所有其他细胞化学染色均为阴性。嗜异性和嗜酸性粒细胞对所有细胞化学染色均呈阳性，但α-NAE除外。嗜碱性粒细胞对POX和AS-D呈强阳性，对PAS和ALP呈阳性，而对SBB，​​ACP和α-NAE染色呈阴性。单核细胞对PAS和α-NAE呈阳性，对ACP呈弱阳性，而对SBB，​​POX，ALP和AS-D染色呈阴性。淋巴细胞对PAS和ACP呈阳性，对AS-D呈弱阳性，而对SBB，​​POX，ALP和α-NAE染色呈阴性。我们的结果增加了有关家鸽血细胞的知识。ACP和α-NAE染色。单核细胞对PAS和α-NAE呈阳性，对ACP呈弱阳性，而对SBB，​​POX，ALP和AS-D染色呈阴性。淋巴细胞对PAS和ACP呈阳性，对AS-D呈弱阳性，而对SBB，​​POX，ALP和α-NAE染色呈阴性。我们的研究结果增加了有关家鸽血细胞的知识。ACP和α-NAE染色。单核细胞对PAS和α-NAE呈阳性，对ACP呈弱阳性，而对SBB，​​POX，ALP和AS-D染色呈阴性。淋巴细胞对PAS和ACP呈阳性，对AS-D呈弱阳性，而对SBB，​​POX，ALP和α-NAE染色呈阴性。我们的结果增加了有关家鸽血细胞的知识。