Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro‑inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1β,TNF-α,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1β and TNF-α in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1β, TNF-α, MCP-1, IL-10, and RANTES, with IL-1β and TNF‑α having the strongest impact.

中文翻译：

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人脂肪组织产生的细胞因子对单核细胞粘附于内皮的影响。

内脏脂肪组织（VAT）可能在动脉粥样硬化性心血管疾病中起关键作用。这项研究的目的是确定人类增值税释放的促炎细胞因子对单核细胞粘附于内皮细胞的作用。使用通过培养人VAT制备的脂肪组织条件培养基（ATCM），测试了细胞因子对单核细胞粘附于内皮细胞（ECs）的影响。测量了刺激的EC中ATCM中的细胞因子浓度，细胞因子表达和粘附分子。在ATCM中测得的IL-1β，TNF-α，MCP-1，IL-10和RANTES浓度与单核细胞对EC的粘附性呈正相关。此外，ATCM增加了粘附分子（ICAM-1，VCAM-1）的基因表达。与没有抑制剂的ATCM预处理相比，选择性抑制剂通过显着降低单核细胞粘附力突出了IL-1β和TNF-α在该过程中的重要性。人增值税显着增加了单核细胞对EC的粘附。它受到IL-1β，TNF-α，MCP-1，IL-10和RANTES的显着影响，其中IL-1β和TNF‑α的影响最大。