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An inexpensive air stream temperature controller and its use to facilitate temperature-controlled behavior in Drosophila.
Biotechniques ( IF 2.7 ) Pub Date : 2019-3-15 , DOI: 10.2144/btn-2018-0152
Ryan Sangston 1 , Jay Hirsh 1
Affiliation  

Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Utilizing flies expressing the temperature-inducible channel dTrpA1 in dopaminergic neurons we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature-modulated biological reagents.

中文翻译:

一种廉价的气流温度控制器及其在果蝇中促进温度控制行为的用途。

控制生物体的环境具有许多生物学相关的应用。事实证明,依赖温度的诱导型生物试剂对于阐明信号级联和神经回路解剖无价。在这里,我们开发了一种简单且价格合理的系统,可以在容纳成年果蝇的室内快速改变温度。利用在多巴胺能神经元中表达温度诱导型通道dTrpA1的果蝇,我们显示了运动行为的快速且可复制的变化。该装置应广泛应用于温度调节的生物试剂。
更新日期:2020-08-21
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