Microorganisms play important roles in the reduction of organic and inorganic pollutants in constructed wetlands used for the treatment of wastewater. However, the diversity and structure of microbial community in constructed wetland system remain poorly known. In this study, the Illumina MiSeq Sequencing of 16S rDNA was used to analyze the bacterial and archaeal microbial community structures of soil and water in a free surface flow constructed wetland, and the differences of bacterial communities and archaeal compositions between soil and water were compared. The results showed that the Proteobacteria were the dominant bacteria, making up 35.38%~48.66% relative abundance. Euryarchaeotic were the absolute dominant archaea in the influent sample with the relative abundance of 93.29%, while Thaumarchaeota showed dominance in the other three samples, making up 50.58%~75.70%. The relative abundances of different species showed great changes in bacteria and archaea, and the number of dominant species in bacteria was much higher than that in archaea. Compared to archaea, the community compositions of bacteria were more abundant and the changes were more significant. Meanwhile, bacteria and archaea had large differences in compositions between water and soil. The microbial richness in water was significantly higher than that in soil. Simultaneously, soil had a significant enrichment effect on some microbial flora.

中文翻译：

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污水处理的完整人工湿地中细菌和古细菌群落结构的变化。

微生物在减少用于处理废水的人工湿地中的有机和无机污染物方面起着重要作用。然而，人工湿地系统中微生物群落的多样性和结构仍然知之甚少。本研究采用16S rDNA的Illumina MiSeq测序方法分析了自由表面流人工湿地中土壤和水的细菌和古细菌微生物群落结构，比较了土壤与水之间细菌群落和古细菌成分的差异。结果表明，变形杆菌为优势菌，相对丰富度为35.38％〜48.66％。Euryarchaeotic是进水样品中的绝对优势古细菌，相对丰度为93.29％，而Thaumarchaeota在其他三个样品中均占优势，占50.58％〜75.70％不同物种的相对丰度表现出细菌和古细菌的巨大变化，细菌中优势种的数量远高于古细菌。与古细菌相比，细菌的群落组成更为丰富，变化也更为明显。同时，细菌和古细菌在水和土壤之间的成分上存在很大差异。水中的微生物丰富度显着高于土壤。同时，土壤对某些微生物菌群具有显着的富集作用。细菌群落组成更丰富，变化更显着。同时，细菌和古细菌在水和土壤之间的成分上存在很大差异。水中的微生物丰富度显着高于土壤。同时，土壤对某些微生物菌群具有显着的富集作用。细菌群落组成更丰富，变化更显着。同时，细菌和古细菌在水和土壤之间的成分上存在很大差异。水中的微生物丰富度显着高于土壤。同时，土壤对某些微生物菌群具有显着的富集作用。