Nutrient metabolism is regulated for adaptation to, for example, environmental alterations, cellular stress, cell cycle, and cellular ageing. This regulatory network consists of cross-talk between cytoplasmic organelles and the nucleus. The ras-like nuclear small G protein, Ran, functions in nuclear-cytosolic transport and regulatory signal transmission. In yeast, some genes involved in the Ran system in yeast are required for growth on glycerol medium. Growth deficiency, due to mutations in the GSP1 gene, which encodes Ran, is allele specific. Specifically in this study, the gsp1-1894 cells lost mitochondria, and could not grow on media containing glycerol, galactose or maltose. However, the gsp1-1894 cells grew better on a high salt medium (1 M NaCl) and had increased expression levels of GPD1-lacZ. Furthermore, disruption of the HOG1 gene suppressed their growth deficiency on glycerol medium. These findings suggest that altered activation of Hog1 in the gsp1-1894 cells resulted in the loss of mitochondria and inhibition of glycerol metabolism. Growth deficiency of the gsp1-1894 cells on galactose medium was further suppressed by high dosage of the SIP2 DNA, which encodes the cytosolic β subunit of AMPK. This suggests that higher cytosolic activity of AMPK is required for the utilization of an alternative carbon source in gsp1-1894 cells.

中文翻译：

---

由于编码酿酒酵母中核小G蛋白的gsp1突变，代谢调控发生改变。

营养代谢被调节以适应例如环境改变，细胞应激，细胞周期和细胞老化。该调节网络由细胞质细胞器和细胞核之间的串扰组成。ras样核小G蛋白Ran在核胞质转运和调节信号传递中起作用。在酵母中，一些与酵母Ran系统有关的基因是在甘油培养基上生长所必需的。由于编码Ran的GSP1基因突变导致的生长缺陷是等位基因特异性的。特别是在这项研究中，gsp1-1894细胞丢失了线粒体，并且无法在含有甘油，半乳糖或麦芽糖的培养基上生长。但是，gsp1-1894细胞在高盐培养基（1 M NaCl）上生长更好，并且GPD1-lacZ的表达水平增加。此外，HOG1基因的破坏抑制了它们在甘油培养基上的生长缺陷。这些发现表明，gsp1-1894细胞中Hog1激活的改变导致线粒体丢失和甘油代谢受到抑制。高剂量的SIP2 DNA可以进一步抑制gsp1-1894细胞在半乳糖培养基上的生长缺陷，该DNA编码AMPK的胞质β亚基。这表明在gsp1-1894细胞中利用替代碳源需要更高的AMPK胞质活性。编码AMPK的胞质β亚基。这表明在gsp1-1894细胞中利用替代碳源需要更高的AMPK胞质活性。编码AMPK的胞质β亚基。这表明在gsp1-1894细胞中利用替代碳源需要更高的AMPK胞质活性。