The spectrophotometric-chemometric analysis of levodopa and carbidopa that are used for Parkinson’s disease was analyzed without any prior reservation. Parkinson’s drugs in the urine sample of a healthy person (never used drugs in his life) were analyzed at the same time spectrophotometrically. The chemometric methods used were partial least squares regression (PLS) and principal component regression (PCR). PLS and PCR were successfully applied as chemometric determination of levodopa and carbidopa in human urine samples. A concentration set including binary mixtures of levodopa and carbidopa in 15 different combinations was randomly prepared in acetate buffer (pH 3.5).). UV spectrophotometry is a relatively inexpensive, reliable, and less time-consuming method. Minitab program was used for absorbance and concentration values. The normalization values for each active substance were good (r2>0.9997). Additionally, experimental data were validated statistically. The results of the analyses of the results revealed high recoveries and low standard deviations. Hence, the results encouraged us to apply the method to drug analysis. The proposed methods are highly sensitive and precise, and therefore they were implemented for the determination of the active substances in the urine sample of a healthy person in triumph.

中文翻译：

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应用化学计量学方法测定人尿中的帕金森氏症药物。

对帕金森氏病使用的左旋多巴和卡比多巴的分光光度化学分析无需事先预约即可进行分析。同时用分光光度法分析健康人尿液中帕金森氏症的药物（他一生中从未使用过药物）。使用的化学计量学方法是偏最小二乘回归（PLS）和主成分回归（PCR）。PLS和PCR已成功应用于人尿样品中左旋多巴和卡比多巴的化学测定。在醋酸盐缓冲液（pH 3.5）中随机制备浓度组，包括左旋多巴和卡比多巴的15种不同组合的二元混合物。紫外分光光度法是一种相对便宜，可靠且耗时较少的方法。Minitab程序用于吸光度和浓度值。每种活性物质的标准化值均良好（r2> 0.9997）。此外，实验数据经过统计学验证。结果分析的结果表明回收率高，标准偏差低。因此，结果鼓励我们将该方法应用于药物分析。所提出的方法高度灵敏且精确，因此可用于测定凯旋健康人尿液中的活性物质。