Abstract Inhalation of aerosols containing pathogenic viruses can result in morbidity, in some cases leading to mortality. The objective of this study was to develop a model for assessing how infectious viruses might distribute in airborne particles using bacteriophage MS2 as a surrogate for human viruses. Particle deposition in the respiratory system is size-dependent, and small virus-containing particles can be inhaled deeply into the lower lungs, potentially leading to more severe respiratory disease manifestations. Laboratory-generated virus-containing particles were size-selected by a differential mobility analyzer and then collected by the newly introduced Super-Efficient Sampler for Influenza Virus. The number of infectious and total viruses per particle as a function of particle size varied with the spraying medium: it approximated a cubic exponential value scaling for deionized (DI) water, a quartic exponential value for artificial saliva (AS), and between quadratic and cubic exponential value for beef extract solution (BES). The survivability of MS2 did not change significantly with particle size for DI water and BES, while that for AS was maximum at 120 nm. Viruses could be homogeneously distributed or aggregated inside or on the surface of the particles, depending on the composition of the spraying medium. Copyright © 2019 American Association for Aerosol Research

中文翻译：

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使用水基冷凝生长技术和噬菌体 MS2 模型确定气溶胶颗粒中传染性病毒的分布

摘要 吸入含有病原病毒的气溶胶会导致发病，在某些情况下甚至导致死亡。本研究的目的是开发一个模型，使用噬菌体 MS2 作为人类病毒的替代品，评估传染性病毒如何在空气传播的颗粒中分布。呼吸系统中的颗粒沉积取决于大小，含有病毒的小颗粒可以被深深吸入下肺，可能导致更严重的呼吸道疾病表现。实验室产生的含病毒颗粒通过差示迁移率分析仪进行尺寸选择，然后由新推出的流感病毒超高效采样器收集。每个颗粒的传染性和总病毒数量作为颗粒尺寸的函数随喷雾介质的变化而变化：它近似于去离子（DI）水的三次指数值缩放，人工唾液（AS）的四次指数值，以及介于二次和牛肉提取物溶液 (BES) 的三次指数值。MS2 的生存能力并没有随着去离子水和 BES 的粒径发生显着变化，而 AS 的生存能力在 120 nm 时达到最大。病毒可以均匀分布或聚集在颗粒内部或表面上，具体取决于喷雾介质的成分。版权所有 © 2019 美国气溶胶研究协会