Spermatogonial stem cells (SSCs) are adult stem cells that are slowly cycling and self‐renewing. The pool of SSCs generates very large numbers of male gametes throughout the life of the individual. SSCs can be cultured in vitro for long periods of time, and established SSC lines can be manipulated genetically. Upon transplantation into the testes of infertile mice, long‐term cultured mouse SSCs can differentiate into fertile spermatozoa, which can give rise to live offspring. Here, we show that the testicular soma of mice with a conditional knockout (conKO) in the X‐linked gene Tsc22d3 supports spermatogenesis and germline transmission from cultured mouse SSCs upon transplantation. Infertile males were produced by crossing homozygous Tsc22d3 floxed females with homozygous ROSA26‐Cre males. We obtained 96 live offspring from six long‐term cultured SSC lines with the aid of intracytoplasmic sperm injection. We advocate the further optimization of Tsc22d3‐conKO males as recipients for testis transplantation of SSC lines.

中文翻译：

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Tsc22d3敲除小鼠的睾丸体支持移植后精原干细胞系的生精和种系传递。

精原干细胞（SSC）是成年的干细胞，它们缓慢循环并自我更新。在整个人的一生中，SSC池会产生大量雄性配子。SSC可以在体外长时间培养，并且已建立的SSC系可以进行基因操作。移植到不育小鼠的睾丸中后，长期培养的小鼠SSC可以分化为可育的精子，从而可以产生活后代。在这里，我们显示了X连锁基因Tsc22d3中有条件敲除（conKO）的小鼠睾丸体在移植后支持培养的小鼠SSC的精子发生和种系传递。不育雄性是通过将纯合的Tsc22d3杂种雌性与纯合的ROSA26-Cre雄性杂交而产生的。我们借助胞浆内精子注射从六个长期培养的SSC系中获得了96个活后代。我们主张进一步优化Tsc22d3-conKO雄性作为SSC系睾丸移植的接受者。