Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.

中文翻译：

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TAF-ChIP：一种超低输入法，用于全基因组染色质免疫沉淀测定。

染色质免疫沉淀（ChIP）和下一代测序（ChIP-Seq）是研究转录调控的强大技术。但是，需要数百万个细胞才能产生具有高信噪比的结果，因此在研究小细胞群时将其排除在外。在这里，我们介绍了一种标记辅助片段化ChIP（TAF-ChIP）和测序方法，可从低细胞数生成高质量的组蛋白图谱。从TAF-ChIP方法获得的数据由于其高信噪比而适合用于ChIP-Seq分析的标准工具。TAF-ChIP方法的表观遗传谱显示与常规ChIP-Seq数据集高度吻合，从而强调了该方法的实用性。