Mosquitoes transmit many harmful diseases that seriously threaten public health. The mosquito’s olfactory system is of great significance for host selection. Inotropic receptors (IRs) and olfactory receptors (ORs) have been demonstrated to be capable of odorant molecular recognition. Analyzing the molecular principles of mosquito olfaction facilitates the development of prevention and therapy techniques. Advances in the understanding of IRs have been seriously inadequate compared to those of ORs. Here, we provide evidence that 35 Anopheles sinensis IR (AsIR) genes are expressed, 7 of which are in the antennae and 2 have expression levels that are upregulated with a blood meal. A homologous analysis of the sequences showed that AsIRs are a subfamily of ionotropic glutamate receptors (iGLURs). This is the first that time IRs have been identified in Anopheles sinensis in vitro. The ultrastructure of the antennae supports the theory that diverse sensilla are distributed in the antennae. The results here may facilitate the revelation of the regulation mechanism in AsIRs, which could mitigate the transmission of diseases by mosquitoes.

中文翻译：

---

在中华按蚊（Diptera：Culicidae）的触角中表达出不同的离子受体。

蚊子传播许多严重危害公共健康的有害疾病。蚊子的嗅觉系统对于选择寄主具有重要意义。正性肌力受体（IRs）和嗅觉受体（ORs）已被证明能够识别气味分子。分析蚊香的分子原理有助于预防和治疗技术的发展。与OR相比，对IR的理解方面的进展严重不足。在这里，我们提供了35个中华按蚊的证据IR（AsIR）基因被表达，其中7个在触角中，而2个的表达水平被血粉上调。序列的同源性分析表明，AsIR是离子型谷氨酸受体（iGLUR）的一个亚家族。这是首次在中华按蚊体外鉴定IR 。触角的超微结构支持了在触角中分布各种感觉的理论。此处的结果可能有助于揭示AsIR中的调节机制，从而可以减轻蚊子传播疾病的机会。