Intermittent hyperbaric oxygen exposure (IE-HBO) can protect the body against oxygen toxicity, but the underlying mechanisms are not very clear. Peroxiredoxin 6 (Prdx6) is a special endogenous antioxidative protein. We explored if the protective effects of IE-HBO are related to Prdx6. Mice were exposed to 280 kPa O2 for 60 min, followed by 30-min exposure to 20% O2/N2 mixture with equal pressure, repeated for six cycles. The Prdx6 protein level and non-selenium glutathione peroxidase (NSGPx) activity in the brain and lungs were then measured and the injury degree of lung and the oxidation level of brain and lung were evaluated. On this basis, the relationship between Prdx6 and IE-HBO's protection was explored. Generally, both IE-HBO and continuous exposure to HBO (CE-HBO) could increase the protein and mRNA levels of Prdx6, and such increases were more significant 24 h after cessation of exposure; moreover, the Prdx6 level of IE-HBO was higher than that of CE-HBO in both brain and lung, also more significantly 24 h after cessation of exposure. In addition, IE-HBO exposure could more effectively potentiate the activity of NSGPx and increase GSH content in brain and lung tissues. At the same time, it could reduce oxidation products in these tissues. IE-HBO could also provide protection for the lungs against injuries resulting from prolonged HBO exposure. These data showed that IE-HBO can potentiate the production and the activity of Prdx6 and consequently mitigate oxidative damages in brain and lungs. The influences of IE-HBO on Prdx6 may form an important basis for its protection against oxygen toxicity.

中文翻译：

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间歇性高压氧暴露可动员过氧化还原蛋白 6 以防止氧中毒。


间歇性高压氧暴露（IE-HBO）可以保护身体免受氧中毒的影响，但其潜在机制尚不清楚。过氧化物酶6（Prdx6）是一种特殊的内源性抗氧化蛋白。我们探讨了 IE-HBO 的保护作用是否与 Prdx6 有关。将小鼠暴露于280 kPa O2 60分钟，然后等压暴露于20% O2/N2混合物30分钟，重复六个循环。测定脑和肺中Prdx6蛋白水平和非硒谷胱甘肽过氧化物酶(NSGPx)活性，评估肺损伤程度以及脑和肺氧化水平。在此基础上探讨了Prdx6与IE-HBO保护的关系。一般情况下，IE-HBO和持续暴露于HBO(CE-HBO)均可增加Prdx6的蛋白和mRNA水平，且这种增加在停止暴露24 h后更为显着；此外，IE-HBO在脑和肺中的Prdx6水平均高于CE-HBO，且在停止暴露24小时后更为显着。此外，IE-HBO暴露可以更有效地增强NSGPx的活性并增加脑和肺组织中的GSH含量。同时，它可以减少这些组织中的氧化产物。 IE-HBO 还可以保护肺部免受长期接触 HBO 造成的伤害。这些数据表明 IE-HBO 可以增强 Prdx6 的产生和活性，从而减轻大脑和肺部的氧化损伤。 IE-HBO对Prdx6的影响可能构成其抗氧中毒的重要基础。