Feeding of adult Ornithodoros tartakovskyi ticks using a modified artificial membrane feeding system.,Medical and Veterinary Entomology

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Feeding of adult Ornithodoros tartakovskyi ticks using a modified artificial membrane feeding system.
Medical and Veterinary Entomology ( IF 1.6 ) Pub Date : 2019-07-25 , DOI: 10.1111/mve.12398
C E Lewis _{1,

2} , L C Bartholomay ₃ , J A Blanchong ₄

Affiliation

Development and maintenance of laboratory tick colonies provides reliable access to a variety of tick species at multiple life stages. Advances in techniques for the membrane feeding of ticks reduce the number of laboratory animals needed for colony maintenance. In the present study, modifications to the existing protocol for in vitro feeding of the argasid species Ornithodoros tartakovskyi were made. Adult O. tartakovskyi ticks of both sexes were allowed to feed to engorgement using a novel membrane feeding apparatus in a six-well plate format with well-inserts of laboratory-grade, wax sealing film. Of the 193 ticks placed on the membrane, 89% (n = 172) fed until engorgement and subsequently detached. The modified feeding method described will aid in future laboratory tick-based research because it allows for increased containment, ease of sorting, successful in vitro feeding, easy replacement of blood meals and a reduction in the total volume of blood meal required.

中文翻译：

使用改良的人工膜饲喂系统饲喂成年Ornithodoros tartakovskyi s。

实验室壁虱菌落的开发和维护可在多个生命阶段提供对各种壁虱物种的可靠访问。tick膜饲喂技术的进步减少了菌落维护所需的实验动物的数量。在本研究中，对现有的拟南芥属物种Ornithodoros tartakovskyi的体外饲养方案进行了修改。使用新颖的膜进料装置，以六孔板格式插入实验级蜡封膜的插口，允许两性的成年O. tartakovskyi tick虫进食。放置在膜上的193个s中，有89％（n = 172）进食直至充血，然后脱离。所描述的改进的喂食方法将有助于将来基于tick的研究，因为它可以增加围堵，

更新日期：2019-07-25

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