FA-SAT is a highly conserved satellite DNA sequence transcribed in many Bilateria species. To disclose the cellular and functional profile of FA-SAT non-coding RNAs, a comprehensive experimental approach, including the transcripts location in the cell and in the cell cycle, the identification of its putative protein interactors, and silencing/ectopic expression phenotype analysis, was performed. FA-SAT non-coding RNAs play a nuclear function at the G1 phase of the cell cycle and the interactomic assay showed that the PKM2 protein is the main interactor. The disruption of the FA-SAT non-coding RNA/PKM2 protein complex, by the depletion of either FA-SAT or PKM2, results in the same phenotype-apoptosis, and the ectopic overexpression of FA-SAT did not affect the cell-cycle progression, but promotes the PKM2 nuclear accumulation. Overall, our data first describe the importance of this ribonucleoprotein complex in apoptosis and cell-cycle progression, what foresees a promising novel candidate molecular target for cancer therapy and diagnosis.

中文翻译：

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FA-SAT ncRNA与PKM2蛋白相互作用：这种复合物的耗尽会导致从细胞增殖转变为凋亡。

FA-SAT是在许多Bilateria物种中转录的高度保守的卫星DNA序列。要公开FA-SAT非编码RNA的细胞和功能概况，一种全面的实验方法，包括转录本在细胞和细胞周期中的位置，其推定的蛋白质相互作用物的鉴定以及沉默/异位表达表型分析，被执行了。FA-SAT非编码RNA在细胞周期的G1期起核功能，相互作用组学分析表明PKM2蛋白是主要的相互作用因子。FA-SAT或PKM2的耗竭破坏了FA-SAT非编码RNA / PKM2蛋白复合物，导致相同的表型凋亡，并且FA-SAT的异位表达不影响细胞周期进步，但促进PKM2核积累。总体，