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Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)
Insect Science ( IF 2.9 ) Pub Date : 2019-07-25 , DOI: 10.1111/1744-7917.12709
Ling Wang 1, 2 , Ying-Dong Bi 1 , Ming Liu 1 , Wei Li 1 , Miao Liu 1 , Shu-Feng Di 1 , Shuai Yang 3 , Chao Fan 1 , Lei Bai 4 , Yong-Cai Lai 1
Affiliation  

The soybean aphid, Aphis glycines, is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding, mating, and foraging. Odorant‐binding proteins (OBPs) play a vital role in olfaction by binding to volatile compounds and by regulating insect sensing of the environment. In this work we used rapid amplification of complementary DNA ends technology to identify and characterize 10 genes encoding A. glycines OBPs (AglyOBPs) belonging to 3 subfamilies, including 4 classic OBPs, 5 Plus‐C OBPs, and one Minus‐C OBP. Quantitative real‐time polymerase chain reaction demonstrated variable specific expression patterns for the 10 genes based on developmental stage and aphid tissue sampled. Expression levels of 7 AglyOBPs (2, 3, 4, 5, 7, 9, and 10) were highest in the 4th instar, indicating that the 4th nymphal instar is an important developmental period during which soybean aphids regulate feeding and search for host plants. Tissue‐specific expression results demonstrated that AglyOBP2, 7, and 9 exhibited significantly higher expression levels in antennae. Meanwhile, ligand‐binding analysis of 5 OBPs demonstrated binding of AglyOBP2 and AglyOBP3 to a broad spectrum of volatiles released by green leaf plants, with bias toward 6‐ to 8‐carbon chain volatiles and strong binding of AglyOBP7 to trans‐β‐farnesene. Taken together, our findings build a foundation of knowledge for use in the study of molecular olfaction mechanisms and provide insights to guide future soybean aphid research.

中文翻译:

大豆蚜虫、甘氨酸蚜虫(半翅目:蚜科)中气味结合蛋白的鉴定和表达谱分析

大豆蚜虫,甘氨酸蚜虫,是一种极端的专家,也是一种重要的入侵性害虫,依靠嗅觉进行觅食、交配和觅食等行为。气味结合蛋白 (OBP) 通过与挥发性化合物结合和调节昆虫对环境的感知,在嗅觉中发挥着至关重要的作用。在这项工作中,我们使用互补 DNA 末端的快速扩增技术来鉴定和表征属于 3 个亚家族的 10 个编码甘氨酸 OBPs (AglyOBPs) 的基因,包括 4 个经典 OBPs、5 个 Plus-C OBPs 和一个 Minus-C OBPs。定量实时聚合酶链反应证明了基于发育阶段和蚜虫组织采样的 10 个基因的可变特异性表达模式。7种AglyOBP(2、3、4、5、7、9和10)的表达水平在4龄期最高,表明第4若虫龄是大豆蚜虫调节摄食和寻找寄主植物的重要发育时期。组织特异性表达结果表明 AglyOBP2、7 和 9 在触角中表现出显着更高的表达水平。同时,对 5 个 OBP 的配体结合分析表明,AglyOBP2 和 AglyOBP3 与绿叶植物释放的广谱挥发物结合,偏向于 6 至 8 碳链挥发物,而 AglyOBP7 与反式β-法呢烯的结合很强。总之,我们的发现为分子嗅觉机制的研究奠定了知识基础,并为指导未来的大豆蚜虫研究提供了见解。组织特异性表达结果表明 AglyOBP2、7 和 9 在触角中表现出显着更高的表达水平。同时,对 5 个 OBP 的配体结合分析表明,AglyOBP2 和 AglyOBP3 与绿叶植物释放的广谱挥发物结合,偏向于 6 至 8 碳链挥发物,而 AglyOBP7 与反式β-法呢烯的结合很强。总之,我们的发现为分子嗅觉机制的研究奠定了知识基础,并为指导未来的大豆蚜虫研究提供了见解。组织特异性表达结果表明 AglyOBP2、7 和 9 在触角中表现出显着更高的表达水平。同时,对 5 个 OBP 的配体结合分析表明,AglyOBP2 和 AglyOBP3 与绿叶植物释放的广谱挥发物结合,偏向于 6 至 8 碳链挥发物,而 AglyOBP7 与反式β-法呢烯的结合很强。总之,我们的发现为分子嗅觉机制的研究奠定了知识基础,并为指导未来的大豆蚜虫研究提供了见解。偏向于 6 到 8 个碳链的挥发物和 AglyOBP7 与反式 β-法呢烯的强结合。总之,我们的发现为分子嗅觉机制的研究奠定了知识基础,并为指导未来的大豆蚜虫研究提供了见解。偏向于 6 到 8 个碳链的挥发物和 AglyOBP7 与反式 β-法呢烯的强结合。总之,我们的发现为分子嗅觉机制的研究奠定了知识基础,并为指导未来的大豆蚜虫研究提供了见解。
更新日期:2019-07-25
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