The genome‐wide characterization of long non‐coding RNA (lncRNA) in insects demonstrates their importance in fundamental biological processes. Essentially, an in‐depth understanding of the functional repertoire of lncRNA in insects is pivotal to insect resources utilization and sustainable pest control. Using a custom bioinformatics pipeline, we identified 1861 lncRNAs encoded by 1852 loci in the Sogatella furcifera genome. We profiled lncRNA expression in different developmental stages and observed that the expression of lncRNAs is more highly temporally restricted compared to protein‐coding genes. More up‐regulated Sogatella furcifera lncRNA expressed in the embryo, 4th and 5th instars, suggesting that increased lncRNA levels may play a role in these developmental stages. We compared the relationship between the expression of Sogatella furcifera lncRNA and its nearest protein gene and found that lncRNAs were more correlated to their downstream coding neighbors on the opposite strand. Our genome‐wide profiling of lncRNAs in Sogatella furcifera identifies exciting candidates for characterization of lncRNAs, and also provides information on lncRNA regulation during insect development.

中文翻译：

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Sogatella furcifera 长链非编码 RNA 的全基因组表征和发育表达谱

昆虫中长链非编码 RNA (lncRNA) 的全基因组表征证明了它们在基本生物过程中的重要性。从本质上讲，深入了解昆虫中 lncRNA 的功能库对于昆虫资源利用和可持续害虫控制至关重要。使用定制的生物信息学管道，我们确定了由 Sogatella furcifera 基因组中的 1852 个基因座编码的 1861 个 lncRNA。我们分析了不同发育阶段的 lncRNA 表达，并观察到 ​​lncRNA 的表达与蛋白质编码基因相比在时间上受到更高的限制。更上调的 Sogatella furcifera lncRNA 在胚胎、第 4 和第 5 龄中表达，表明 lncRNA 水平增加可能在这些发育阶段发挥作用。我们比较了 Sogatella furcifera lncRNA 的表达与其最近的蛋白质基因之间的关系，发现 lncRNA 与其下游编码邻居的相反链更相关。我们对 Sogatella furcifera 中 lncRNA 的全基因组分析确定了令人兴奋的 lncRNA 表征候选者，并提供了昆虫发育过程中 lncRNA 调控的信息。