BACKGROUND Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible. RESULTS In this regard, two DnaJs (Cn-DnaJa1 and Cn-DnaJb1) are cloned from the Coilia nasus's ovary. Their structure both contains J domain, G/F domain and ZF domain. Their mRNA transcripts were found extensively expressed in all the sampled tissues and significantly highly in gonads, which probably mean that DnaJs involved in C. nasus's gonad development basal metabolic processes. In the process of spawning migration, Cn-DnaJa1 and Cn-DnaJb1 mRNA transcripts were also expressed with significant differences during oogenesis with highest levels in the development phase, and maintaining high levels during the multiplication, mature and spawning phase. Further study showed that the DnaJa1and DnaJb1protein have high distribution in the onset phase and mainly distributed in the oocyte cytoplasm especially during the migration development phase's. CONCLUSIONS This experiment study demonstrated that DnaJs participate in reproductive regulation during the spawning migration process in C. nasus and possibly play a vital role in the ovary development process. These findings also provided a base knowledge for further molecular mechanism study of spawning migration.

中文翻译：

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Co毛中DnaJa1和DnaJb1的分子克隆和特征：产卵迁移过程中可能与卵子发生有关。

背景技术Co虫的卵子发生/产卵迁移是一个明确定义的同步安排过程。DnaJs是卵子发生过程中必不可少的分子伴侣。然而，DnaJs如何参与异常产卵迁移机制是杰出且合理的。结果在这方面，从Coilia nasus的卵巢中克隆了两个DnaJ（Cn-DnaJa1和Cn-DnaJb1）。它们的结构都包含J域，G / F域和ZF域。他们的mRNA转录物在所有采样的组织中广泛表达，在性腺中显着高表达，这可能意味着DnaJs参与了鼻息肉的性腺发育基础代谢过程。在产卵迁移过程中，Cn-DnaJa1和Cn-DnaJb1 mRNA转录本在成卵过程中也有显着差异，在发育阶段处于最高水平，并在繁殖，成熟和产卵阶段保持较高水平。进一步的研究表明，DnaJa1和DnaJb1蛋白在发病期具有较高的分布，主要分布在卵母细胞的细胞质中，特别是在迁移发育阶段。结论这项实验研究表明DnaJs在鼻息肉的产卵迁移过程中参与生殖调节，并且可能在卵巢发育过程中起着至关重要的作用。这些发现也为产卵迁移的进一步分子机理研究提供了基础知识。进一步的研究表明，DnaJa1和DnaJb1蛋白在发病期具有较高的分布，主要分布在卵母细胞的细胞质中，特别是在迁移发育阶段。结论这项实验研究表明DnaJs在鼻息肉的产卵迁移过程中参与生殖调节，并且可能在卵巢发育过程中起着至关重要的作用。这些发现还为产卵迁移的进一步分子机理研究提供了基础知识。进一步的研究表明，DnaJa1和DnaJb1蛋白在发病期具有较高的分布，主要分布在卵母细胞的细胞质中，特别是在迁移发育阶段。结论这项实验研究表明DnaJs在鼻息肉的产卵迁移过程中参与生殖调节，并且可能在卵巢发育过程中起着至关重要的作用。这些发现也为产卵迁移的进一步分子机理研究提供了基础知识。