BACKGROUND Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible. RESULTS In this regard, two DnaJs (Cn-DnaJa1 and Cn-DnaJb1) are cloned from the Coilia nasus's ovary. Their structure both contains J domain, G/F domain and ZF domain. Their mRNA transcripts were found extensively expressed in all the sampled tissues and significantly highly in gonads, which probably mean that DnaJs involved in C. nasus's gonad development basal metabolic processes. In the process of spawning migration, Cn-DnaJa1 and Cn-DnaJb1 mRNA transcripts were also expressed with significant differences during oogenesis with highest levels in the development phase, and maintaining high levels during the multiplication, mature and spawning phase. Further study showed that the DnaJa1and DnaJb1protein have high distribution in the onset phase and mainly distributed in the oocyte cytoplasm especially during the migration development phase's. CONCLUSIONS This experiment study demonstrated that DnaJs participate in reproductive regulation during the spawning migration process in C. nasus and possibly play a vital role in the ovary development process. These findings also provided a base knowledge for further molecular mechanism study of spawning migration.

中文翻译：

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鲚DnaJa1和DnaJb1的分子克隆和特征：在产卵迁徙过程中参与卵子发生的可能功能。


背景技术鲚卵子发生/产卵迁移是一个明确的同步排列过程。 DnaJ 是卵子发生过程中不可或缺的分子伴侣。然而，DnaJs 如何参与溯河产卵迁移机制是突出且合理的。结果 在这方面，从鲚的卵巢中克隆了两个 DnaJ（Cn-DnaJa1 和 Cn-DnaJb1）。它们的结构均包含J结构域、G/F结构域和ZF结构域。他们的 mRNA 转录本在所有采样组织中广泛表达，并且在性腺中显着高表达，这可能意味着 DnaJ 参与了 C. nasus 性腺发育的基础代谢过程。在产卵洄游过程中，Cn-DnaJa1和Cn-DnaJb1 mRNA转录本在卵子发生过程中也有显着差异，在发育期表达量最高，在增殖期、成熟期和产卵期保持高水平。进一步研究表明，DnaJa1和DnaJb1蛋白在卵母细胞发生期有较高的分布，尤其是在迁移发育期主要分布在卵母细胞胞质中。结论本实验研究表明，DnaJs参与了C. nasus产卵迁移过程中的生殖调控，并可能在卵巢发育过程中发挥着重要作用。这些发现也为进一步研究产卵迁移的分子机制提供了基础知识。