Purpose: To develop peripheral blood mRNA expression profiles of drug metabolizing enzymes (DMEs) as a surrogate to monitor tobacco induced head and neck squamous cell carcinoma (HNSCC), attempts were made to investigate (i) similarities in alterations with the cancer marker genes in biopsy samples and (ii) if alterations similar to that seen in biopsy samples are reflected in peripheral blood. Methods: Total RNA from eight soft gingival tissues and eight biopsy samples of HNSCC patients and total DNA and RNA from blood of healthy controls (n = 150) and HNSCC patients (n = 150) was processed for expression and genotyping studies. Blood from patients receiving chemo-radiotherapy was processed for follow-up study. Results: qRT-PCR revealed significant increase in mRNA expression of DMEs in biopsy and blood samples of HNSCC patients when compared to controls. Similar alterations were observed in cancer marker genes in these samples. Patients with variant genotypes of DMEs showed greater magnitude of alterations in mRNA expression when compared to wild type controls. Responders of chemo-radiotherapy showed significant decline in induction of mRNA expression of DMEs and cancer marker genes Conclusions: The data suggest that peripheral blood expression profiles could be used to monitor tobacco-induced HNSCC as well as the treatment response.

中文翻译：

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外周血药物代谢酶和癌症标志物基因的mRNA表达与头颈癌患者的活检样本相似。

目的：建立药物代谢酶（DMEs）的外周血mRNA表达谱，以监测烟草诱发的头颈鳞状细胞癌（HNSCC），试图研究（i）与癌症标记基因的相似性。活检样本和（ii）如果与活检样本相似的变化反映在外周血中。方法：对来自HNSCC患者的8个软牙龈组织和8个活检样本的总RNA以及健康对照（n = 150）和HNSCC患者（n = 150）血液中的总DNA和RNA进行表达和基因分型研究。对接受化学放射治疗的患者的血液进行处理以进行后续研究。结果：与对照组相比，qRT-PCR显示HNSCC患者的活检和血液样本中DME的mRNA表达显着增加。在这些样品中的癌症标记基因中观察到类似的变化。与野生型对照相比，具有DME变异基因型的患者显示出更大的mRNA表达变化。化学放疗反应者显示DMEs和癌症标志物基因的mRNA表达诱导显着下降结论：数据表明外周血表达谱可用于监测烟草诱导的HNSCC以及治疗反应。