Common histopathologic techniques are used to diagnose fungal infections, but the diagnostic identification of mycoses in tissue specimens is often difficult, particularly when fungi rarely occur in a specimen. The aim of this study was to evaluate the application of fluorescein-labeled chitinase staining to formalin-fixed and paraffin-embedded (FFPE) tissues. We studied 79 archival FFPE tissues from patients diagnosed with fungal disease, including 38 cases of sporotrichosis and 41 cases of other fungal infections. The tissue sections were subjected to periodic acid-Schiff (PAS) staining, Gomori's methenamine silver (GMS) staining, and fluorescein-labeled chitinase staining to detect fungal elements. Culture- and/or hematoxylin-eosin-positive samples were used to estimate the diagnostic sensitivity of each staining method, with the results showing that PAS, GMS, and fluorescein-labeled chitinase staining had sensitivities of 50.6, 70.9, and 68.4%, respectively. The three staining results were the same for all fungal infections except for sporotrichosis and chromoblastomycosis. Fluorescein-labeled chitinase staining exhibited high sensitivity in cases of sporotrichosis and poor performance in detecting muriform cells of chromoblastomycosis. On the whole, the sensitivity of fluorescein-labeled chitinase staining was greater than that of PAS and similar to that of GMS staining. Therefore, the results of our study suggest that fluorescein-labeled chitinase staining is a potentially useful diagnostic tool in the diagnosis of fungal infections.

中文翻译：

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荧光素标记的几丁质酶染色对福尔马林固定和石蜡包埋的真菌病组织的诊断价值。

常用的组织病理学技术可用于诊断真菌感染，但是组织标本中真菌病的诊断鉴定通常很困难，尤其是当真菌很少在标本中发生时。这项研究的目的是评估荧光素标记的几丁质酶染色在福尔马林固定和石蜡包埋（FFPE）组织中的应用。我们研究了诊断为真菌病患者的79份FFPE组织，其中包括38例孢子体病和41例其他真菌感染。对组织切片进行高碘酸-希夫（PAS）染色，Gomori的二甲胺银（GMS）染色和荧光素标记的几丁质酶染色，以检测真菌成分。培养和/或苏木精-曙红阳性样品用于评估每种染色方法的诊断敏感性，结果表明，PAS，GMS和荧光素标记的几丁质酶染色的灵敏度分别为50.6％，70.9％和68.4％。除孢子菌病和成色母细胞病外，所有真菌感染的三种染色结果均相同。荧光素标记的几丁质酶染色在孢子体增生的情况下显示出高灵敏度，并且在色母细胞病的粘膜样细胞的检测中表现较差。总体而言，荧光素标记的几丁质酶染色的敏感性高于PAS的敏感性，与GMS染色的敏感性相似。因此，我们的研究结果表明，荧光素标记的几丁质酶染色是诊断真菌感染的潜在有用的诊断工具。除孢子菌病和成色母细胞病外，所有真菌感染的三种染色结果均相同。荧光素标记的几丁质酶染色在孢子体增生的情况下显示出高灵敏度，并且在色母细胞病的粘膜样细胞的检测中表现较差。总体而言，荧光素标记的几丁质酶染色的敏感性高于PAS的敏感性，与GMS染色的敏感性相似。因此，我们的研究结果表明，荧光素标记的几丁质酶染色是诊断真菌感染的潜在有用的诊断工具。除孢子菌病和成色母细胞病外，所有真菌感染的三种染色结果均相同。荧光素标记的几丁质酶染色在孢子体增生的情况下显示出高灵敏度，并且在色母细胞病的粘膜样细胞的检测中表现较差。总体而言，荧光素标记的几丁质酶染色的敏感性高于PAS的敏感性，与GMS染色的敏感性相似。因此，我们的研究结果表明，荧光素标记的几丁质酶染色是诊断真菌感染的潜在有用的诊断工具。荧光素标记的几丁质酶染色在孢子体增生的情况下显示出高灵敏度，并且在色母细胞病的粘膜样细胞的检测中表现较差。总体而言，荧光素标记的几丁质酶染色的敏感性高于PAS的敏感性，与GMS染色的敏感性相似。因此，我们的研究结果表明，荧光素标记的几丁质酶染色是诊断真菌感染的潜在有用的诊断工具。荧光素标记的几丁质酶染色在孢子体增生的情况下显示出高灵敏度，并且在色母细胞病的粘膜样细胞的检测中表现较差。总体而言，荧光素标记的几丁质酶染色的敏感性高于PAS的敏感性，与GMS染色的敏感性相似。因此，我们的研究结果表明，荧光素标记的几丁质酶染色是诊断真菌感染的潜在有用的诊断工具。