A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. The anti-chloramphenicol antibody (mAb) adopted in this work for direct immunoassay could bind to CAP specifically, with negligible cross-reactivity (CR) (less than 0.01%) with most CAP analogues, including structurally related thiamphenicol (TAP) and florfenicol (FF). The limit of detection (LOD), measured by IC₁₀, was 0.0021 ng mL⁻¹. The detection range (IC₂₀-IC₈₀) was ranged from 0.00979 to 0.12026 ng mL⁻¹. In spiked cosmetics samples, mean recoveries ranged from 82.7% to 99.6%, with intraday and interday variation less than 9.8 and 8.2%, respectively. Moreover, with the help of HRP-labeled anti-CAP mAb, the method could be processed in fast direct immunoreaction mode. This CL-ELISA method could be applied for specific, rapid, semiquantitative, and quantitative detection of CAP in cosmetics, facilitating the precise quality control of CAP contamination.

中文翻译：

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用于化妆品中氯霉素测定的高特异性化学发光免疫分析法。

已经开发了一种直接和高度特异性的化学发光酶联免疫吸附测定（CL-ELISA）方法，用于监测化妆品中的氯霉素（CAP）。在这项工作中采用的用于直接免疫分析的抗氯霉素抗体（mAb）可以与CAP特异性结合，与大多数CAP类似物（包括与结构相关的甲砜霉素（TAP）和氟苯尼考（ FF）。通过IC ₁₀测量的检出限（LOD）为0.0021ngmL ^-1。检测范围（IC ₂₀ -IC ₈₀）中的范围为0.00979至0.12026纳克毫升^-1。在加标的化妆品样品中，平均回收率在82.7％至99.6％之间，日内和日间差异分别小于9.8和8.2％。此外，借助HRP标记的抗CAP mAb，可以在快速直接免疫反应模式下处理该方法。这种CL-ELISA方法可用于化妆品中CAP的特异性，快速，半定量和定量检测，从而有助于CAP污染的精确质量控制。