Fat deposition in muscle includes intramuscular fat (IMF) and intermuscular fat. IMF content is an index of pork quality; however, because IMF content is difficult to measure in vivo in young animals, conventional breeding for IMF content is difficult to carry out. The mechanism and progression of animal fat deposition is not well understood, and there are currently no effective control methods. In this study, using Laiwu and large white pigs as the research subjects and RNA sequencing technology, we analyzed the genetic mechanism of animal fat deposition in pigs. Specifically, we analyzed the features of lncRNAs and their potential target genes. We obtained 464 million clean reads, from which 907 lncRNAs were identified. The cis and trans analysis identified target genes, including genes that were upregulated (286) and downregulated (621) in the fatty and lean pigs. ENSSSCG00000008692_ADD1, ENSSSCG00000023124_ADD1 and ENSSSCG00000005918_DGAT1 were validated as target genes of the lncRNAs and were shown to be closely related to fat deposition. These results provide a basis for studying the different metabolic lncRNA expression of IMF deposition. In addition, as the valuable model animal to study the mechanisms of obesity, pigs may represent a new avenue for studying human obesity.

中文翻译：

---

高通量测序对脂肪和瘦肉猪脂肪沉积的分子机理的分析和初步验证。

肌肉中的脂肪沉积包括肌内脂肪（IMF）和肌间脂肪。IMF含量是猪肉质量的指标；但是，由于在幼小的动物体内难以测量IMF含量，因此难以进行IMF含量的常规育种。动物脂肪沉积的机制和进展尚不十分清楚，目前还没有有效的控制方法。本研究以莱芜市大型白猪为研究对象，采用RNA测序技术，分析了猪体内脂肪沉积的遗传机制。具体来说，我们分析了lncRNA的特征及其潜在的靶基因。我们获得了4.64亿个干净读取，从其中识别了907个lncRNA。顺式和反式分析确定了目标基因，包括在肥大和瘦肉猪中上调的基因（286）和下调的基因（621）。ENSSSCG00000008692_ADD1，ENSSSCG00000023124_ADD1和ENSSSCG00000005918_DGAT1被确认为lncRNA的靶基因，并被证明与脂肪沉积密切相关。这些结果为研究IMF沉积的不同代谢lncRNA表达提供了基础。另外，作为研究肥胖机理的有价值的模型动物，猪可能代表了研究人类肥胖的新途径。