Background: Adult pancreatic beta cells, though quiescent, can proliferate in response to physiological need. This inherent character is used in exploring the possibilities of expanding the beta cell mass in the treatment of diabetes. Forkhead box M1 (FoxM1) transcription factor is an important regulator in the proliferation and survival of adult beta cell mass. Naringin, a flavanone glycoside, is reported to have antidiabetic activity and exhibited an increase in insulin levels in diabetic animals. Objectives: The present study tried to evaluate the role of naringin in the regulation of FoxM1 in the pancreas of diabetic rats and to reascertain its antilipidemic and antioxidant properties. Methods: Diabetes was induced in male rats using streptozotocin and treated with naringin (100 mg/kg) orally for 4 and 8 weeks. Serum biochemical parameters, insulin, gene and protein expression of FoxM1, and antioxidant markers in rat pancreas were analyzed. Results: Naringin administration reduced the blood sugar, urea, creatinine, and cholesterol values and improved the pancreatic antioxidant status in diabetic rats. Naringin-treated diabetic rats showed a significant increase in mRNA and protein expression of FoxM1 compared to the diabetic control rats, indicating regeneration of cells. It also increased the insulin immunopositive cells, indicating functional beta cells. Conclusion: Naringin was found to upregulate the FoxM1 transcription factor in diabetic animals, which influenced the proliferation and functional status of beta cells.

中文翻译：

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柚皮苷通过 Forkhead Box M1 介导的 β 细胞增殖改善链脲佐菌素诱导的糖尿病

背景：成人胰腺 β 细胞虽然处于静止状态，但可以根据生理需要增殖。这种固有特性用于探索在糖尿病治疗中扩大 β 细胞群的可能性。Forkhead box M1 (FoxM1) 转录因子是成人 β 细胞群增殖和存活的重要调节因子。据报道，柚皮苷是一种黄烷酮糖苷，具有抗糖尿病活性，并能增加糖尿病动物的胰岛素水平。目的：本研究试图评估柚皮苷在调节糖尿病大鼠胰腺中 FoxM1 中的作用，并重新确定其抗血脂和抗氧化特性。方法：使用链脲佐菌素在雄性大鼠中诱导糖尿病，并用柚皮苷 (100 mg/kg) 口服治疗 4 和 8 周。血清生化参数，分析了大鼠胰腺中的胰岛素、FoxM1 基因和蛋白质表达以及抗氧化标志物。结果：柚皮苷降低了糖尿病大鼠的血糖、尿素、肌酐和胆固醇值，并改善了胰腺抗氧化状态。与糖尿病对照大鼠相比，柚皮苷处理的糖尿病大鼠显示出 FoxM1 的 mRNA 和蛋白质表达显着增加，表明细胞再生。它还增加了胰岛素免疫阳性细胞，表明有功能的β细胞。结论：发现柚皮苷可上调糖尿病动物的 FoxM1 转录因子，从而影响 β 细胞的增殖和功能状态。肌酐和胆固醇值，并改善糖尿病大鼠的胰腺抗氧化状态。与糖尿病对照大鼠相比，柚皮苷处理的糖尿病大鼠显示出 FoxM1 的 mRNA 和蛋白质表达显着增加，表明细胞再生。它还增加了胰岛素免疫阳性细胞，表明功能性β细胞。结论：发现柚皮苷可上调糖尿病动物的 FoxM1 转录因子，从而影响 β 细胞的增殖和功能状态。肌酐和胆固醇值，并改善糖尿病大鼠的胰腺抗氧化状态。与糖尿病对照大鼠相比，柚皮苷处理的糖尿病大鼠显示出 FoxM1 的 mRNA 和蛋白质表达显着增加，表明细胞再生。它还增加了胰岛素免疫阳性细胞，表明功能性β细胞。结论：发现柚皮苷可上调糖尿病动物的 FoxM1 转录因子，从而影响 β 细胞的增殖和功能状态。表示功能性β细胞。结论：发现柚皮苷可上调糖尿病动物的 FoxM1 转录因子，从而影响 β 细胞的增殖和功能状态。表示功能性β细胞。结论：发现柚皮苷可上调糖尿病动物的 FoxM1 转录因子，从而影响 β 细胞的增殖和功能状态。