Colorectal cancer has a common cause of morbidity and mortality. Therefore, it is urgent to detect reliable biomarkers to predict prognosis in CRC. Here, we determined the expression of TIMP-2 and MMP-9 in a CRC tissue microarray by immunohistochemistry. We found that lower TIMP-2 or/and higher MMP-9 expression in cancer tissues was correlated with poorer overall survival (OS). TIMP-2 or MMP-9 expression was independent prognostic factors for CRC. Furthermore, TIMP-2 and MMP-9 expression had a synergistic role as efficient prognostic indicators for CRC patients. In vitro and in vivo, TIMP-2 could inhibit HCT 116 cells invasion and migration by regulating MMP-9. In sum, a combined expression of TIMP-2 and MMP-9 as efficient prognostic indicators was found for the first time.

中文翻译：

---

TIMP-2通过调节MMP-9抑制转移并预测结直肠癌的预后。

大肠癌是发病和死亡的常见原因。因此，迫切需要检测可靠的生物标志物以预测CRC的预后。在这里，我们通过免疫组织化学确定了TIMP-2和MMP-9在CRC组织微阵列中的表达。我们发现癌症组织中较低的TIMP-2或/和较高的MMP-9表达与较差的总体生存率（OS）相关。TIMP-2或MMP-9表达是CRC的独立预后因素。此外，TIMP-2和MMP-9表达作为CRC患者的有效预后指标具有协同作用。在体外和体内，TIMP-2可以通过调节MMP-9抑制HCT 116细胞的侵袭和迁移。总之，首次发现了TIMP-2和MMP-9的联合表达作为有效的预后指标。