Chronic wasting disease (CWD) agents are shed into biological samples, facilitating their horizontal transmission between cervid species. Once prions enter the environment, binding of PrP^CWD by soil particles may maintain them near the soil surface, posing a challenge for decontamination. A 2 N sodium hydroxide (NaOH) or 2% sodium hypochlorite (NaClO) solution is traditionally recommended for prion decontamination of equipment and surfaces. Using protein misfolding cyclic amplification with beads and a bioassay with TgElk mice, we compared the effects of these disinfectants in CWD-contaminated soil for 1 or 16 h to those of controls of known infectious titres. Our results suggest that 2 N NaOH in a 1/5 farm soil volume provides a large decrease (>10²-fold) in prion infectivity.

慢性消耗性疾病（CWD）病菌会掉入生物样品中，从而促进其在宫颈物种之间的水平传播。一旦病毒进入环境，土壤颗粒对PrP ^CWD的结合作用可能使它们保持在土壤表面附近，这给净化带来了挑战。传统上建议使用2 N氢氧化钠（NaOH）或2％次氯酸钠（NaClO）溶液对设备和表面进行病毒去污。使用珠蛋白折叠错误的循环扩增和TgElk小鼠的生物测定，我们将这些消毒剂在受CWD污染的土壤中1或16 h的效果与已知感染滴度的对照进行了比较。我们的结果表明，在1/5的农田土壤中添加2 N NaOH可使病毒的感染力大大降低（> 10 ²倍）。