In this review, we discuss applications of flow cytometric immunophenotyping (FCI) in the diagnostic evaluation and posttreatment monitoring of B and T lymphoblastic leukemia/lymphoma. We describe practical approaches to FCI at the time of diagnosis, with an emphasis on blast identification, lineage assignment, and distinction of B and T lymphoblastic leukemia/lymphoma from their morphologic and immunophenotypic mimics. We further review flow cytometric assays for the detection of minimal or measurable residual disease (MRD) after treatment, and illustrate both standard approaches, and newer strategies for improving sensitivity and circumventing the loss of immunophenotypic targets after immunotherapy. © 2019 International Clinical Cytometry Society.

中文翻译：

---

流式细胞术免疫分型在B和T淋巴细胞白血病/淋巴瘤的诊断和后处理监测中的应用。

在这篇综述中，我们讨论了流式细胞仪免疫表型（FCI）在B和T淋巴细胞白血病/淋巴瘤的诊断评估和治疗后监测中的应用。我们在诊断时描述了FCI的实用方法，重点是胚细胞鉴定，谱系分配以及B和T淋巴母细胞白血病/淋巴瘤的形态学和免疫表型模拟物区分。我们进一步审查了流式细胞仪检测治疗后最小或可测量的残留疾病（MRD）的检测方法，并说明了标准方法和新方法，以提高免疫治疗后的敏感性并避免免疫表型靶标的丢失。©2019国际临床细胞计量学会。