A widely recognized limitation in mammalian olfactory research is the lack of current methods for measuring odor availability (i.e., the quantifiable amount of odor presented and thus available for olfaction) of training or testing materials during behavioral or operational testing. This research utilized an existing technology known as Controlled Odor Mimic Permeation Systems (COMPS) to produce a reproducible, field-appropriate odor delivery method that can be analytically validated and quantified, akin to laboratory-based research methods, such as permeation devices that deliver a stable concentration of a specific chemical vapor for instrumental testing purposes. COMPS were created for 12 compounds across a range of carbon chain lengths and functional groups in such a way to produce similar permeation rates for all compounds. Using detection canines as a model, field-testing was performed to assess the efficacy of the method. Additionally headspace concentrations over time were measured as confirmation of odor availability using either externally sampled internal standard-solid phase microextraction-gas chromatography-mass spectrometry (ESIS-SPME-GC-MS) or collection onto a programmable temperature vaporizing (PTV) GC inlet with MS detection. Finally, lifetime usage was considered. An efficient method for producing and measuring reliable odor availabilities across various chemical functional groups was developed, addressing a noted gap in existing literature that will advance canine and other nonhuman mammal research testing.

中文翻译：

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嗅觉现场测试中受控的气味传递方法。

在哺乳动物嗅觉研究中，一个公认的局限性是缺乏在行为或操作测试过程中测量训练或测试材料的气味可用性（即，所呈现气味的可量化数量，因此可用于嗅觉）的当前方法。这项研究利用一种称为受控气味模拟渗透系统（COMPS）的现有技术，产生了一种可重现的，适合于现场的气味释放方法，该方法可以进行分析验证和量化，类似于基于实验室的研究方法，例如将污染物释放到人体的渗透装置。用于仪器测试的特定化学蒸气的稳定浓度。针对碳链长度和官能团范围内的12种化合物创建了COMPS，以使所有化合物产生相似的渗透率。使用检测犬作为模型，进行了现场测试以评估该方法的有效性。另外，使用外部取样的内部标准固相微萃取-气相色谱-质谱（ESIS-SPME-GC-MS）或收集到带有温度的可编程温度汽化（PTV）GC进样口中，测量一段时间内的顶空浓度，以确认是否有气味。 MS检测。最后，考虑了终生使用情况。开发了一种有效的方法来产生和测量各种化学官能团之间可靠的气味，以解决现有文献中指出的空白，这些空白将促进犬类和其他非人类哺乳动物的研究测试。另外，使用外部采样的内部标准固相微萃取-气相色谱-质谱（ESIS-SPME-GC-MS）或收集到带有温度的可编程温度蒸发（PTV）GC进样口中，测量一段时间内的顶空浓度，以确认是否有气味。 MS检测。最后，考虑了终生使用情况。开发了一种有效的方法来产生和测量各种化学官能团之间可靠的气味，以解决现有文献中指出的空白，这些空白将促进犬类和其他非人类哺乳动物的研究测试。另外，使用外部取样的内部标准固相微萃取-气相色谱-质谱（ESIS-SPME-GC-MS）或收集到带有温度的可编程温度汽化（PTV）GC进样口中，测量一段时间内的顶空浓度，以确认是否有气味。 MS检测。最后，考虑了终生使用情况。开发了一种有效的方法来产生和测量各种化学官能团之间可靠的气味，以解决现有文献中指出的空白，这些空白将促进犬类和其他非人类哺乳动物的研究测试。