Caenorhabditis elegans is an invertebrate model organism used in many areas of biology including developmental biology and the identification of molecular mechanisms and pathways. However, several experimental approaches require large quantities of worms, which is limiting and time-consuming. We present a protocol that uses modern fermentation methodology to effectively produce large numbers of C. elegans using a 7-l bioreactor in a fed-batch cultivation procedure. The production is modular and flexible as well as being a self-controlled system, thus not much labor is required until harvesting C. elegans. The high-yield worm cultivation is flexible and simple to amend, and now allows for the extended application of C. elegans as a model organism and expression system, including large-scale protein production.

中文翻译：

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使用劳动友好的分批补料方法在生物反应器中大规模培养秀丽隐杆线虫。

秀丽隐杆线虫是一种无脊椎动物模型生物，用于许多生物学领域，包括发育生物学以及分子机制和途径的鉴定。但是，一些实验方法需要大量的蠕虫，这是有限且耗时的。我们提出了一种协议，该协议使用现代发酵方法在补料分批培养程序中使用7-1生物反应器有效生产大量秀丽隐杆线虫。生产是模块化，灵活的，并且是一个自控系统，因此在收获秀丽隐杆线虫之前不需要太多的劳动力。高产蠕虫的栽培灵活且易于修改，现在可以扩展秀丽隐杆线虫的应用 作为模型有机体和表达系统，包括大规模蛋白质生产。