The quail-chick chimera marking system, devised in 1969, gave a new impetus to the analysis of cell migrations and interactions in the developing nervous, immune and hematopoietic systems. The method is based on the observation that the constitutive heterochromatin in all embryonic and adult cells of the quail is condensed in one large mass in the centre of the nucleus and is associated with the nucleolus, making this organelle strongly stained with the Feulgen-Rossenbeck reaction. The association of cells or rudiments from two avian species, advocated as a means to identify cells that migrate during embryogenesis, was rapidly recognized in this context as a useful tool for the study of many developmental biology problems. This article summarizes the fundamental contribution of Nicole Le Douarin to the discovery and the application of this technique over the last 40 years.

中文翻译：

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Nicole Le Douarin 和使用鹌鹑小鸡嵌合体研究神经嵴和造血细胞的发育命运

1969 年设计的鹌鹑-小鸡嵌合体标记系统为分析发育中的神经、免疫和造血系统中的细胞迁移和相互作用提供了新的动力。该方法基于以下观察：鹌鹑所有胚胎和成体细胞中的组成型异染色质在细胞核中心凝聚成一大团，并与核仁结合，使该细胞器被 Feulgen-Rossenbeck 反应强烈染色. 来自两个鸟类物种的细胞或雏形的关联，被提倡作为识别胚胎发生过程中迁移的细胞的一种手段，在这种情况下迅速被认为是研究许多发育生物学问题的有用工具。