In the last decades, biodegradation as an environmentally friendly approach has raised interest in connection with the removal of hydrocarbon pollutants. Its capacity for removing pollutants strongly depends on the type of living cell and environmental conditions. The degradative activity of a new sophorolipid-producing yeast, Candida catenulata KP324968, in the removal of high concentrations of diesel from effluents was statistically evaluated considering the initial pH, the agitation speed, and the initial diesel concentration. The optimal setting of the operational variables at an initial pH of 4.7, an agitation speed of 204 rpm, and an initial diesel concentration of 93.4 g L-1 resulted in the highest total petroleum hydrocarbon removal efficiency: about 82.1% after 6 days (biodegradation rate: 0.378 g gcell-1 h-1). During the cell growth phase, the emulsification index in the medium increased and reached its highest level at 64.6% after 48 h. Further tests indicated that the emulsification capacity was obtained by in situ production of two sophorolipid molecules with an m/z of 533 and 583. In summary, its effective diesel removal and high emulsification capacity makes C. catenulata KP324968 an attractive candidate yeast for the degradation of hydrocarbons from aqueous environments.

中文翻译：

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生产槐糖脂的酵母假丝酵母KP324968在高浓度下快速生物降解柴油中的烃。

在过去的几十年中，生物降解作为一种环境友好的方法引起了人们对去除碳氢化合物污染物的兴趣。其去除污染物的能力在很大程度上取决于活细胞的类型和环境条件。考虑到初始pH，搅拌速度和初始柴油浓度，从统计学上评估了产生新槐糖脂的酵母Candida catenulata KP324968去除废水中高浓度柴油的降解活性。在初始pH值为4.7，搅拌速度为204 rpm和初始柴油浓度为93.4 g L-1的情况下，对操作变量进行最佳设置可实现最高的总石油烃去除效率：6天后约82.1％（生物降解）速率：0.378 g gcell-1 h-1）。在细胞生长阶段，培养基中的乳化指数增加，并在48小时后达到最高水平，为64.6％。进一步的测试表明，乳化能力是通过原位生产两个m / z为533和583的槐糖脂分子获得的。总的来说，其有效的柴油去除率和高乳化能力使C. catenulata KP324968成为降解的有吸引力的候选酵母。来自水性环境的碳氢化合物。