In this study, in order to directly and efficiently convert inulin into a single-cell oil (SCO), an INU1 gene encoding inulinase from Kluyveromyces marxianus was integrated into the genomic DNA and actively expressed in an SCO producer Aureobasidium melanogenum P10. The transformant API41 obtained produced 28.5 U/mL of inulinase and its wild-type strain P10 yielded only 8.62 U/mL. Most (97.5%) of the inulinase produced by the transformant API41 was secreted into the culture. During a 10-L fermentation, 66.2% (w/w) lipid in the yeast cells of the transformant API41 and 14.38 g/L of cell dry weight were attained from inulin of 80.0 g/L within 120 h, high inulinase activity (23.7 U/mL) was also produced within 72 h, and the added inulin was actively hydrolyzed. This confirmed that the genetically engineered yeast of A. melanogenum P10 is suitable for direct production of lipids from inulin. The lipids produced could be used as feedstocks for biodiesel production.

中文翻译：

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菊粉中的菊粉酶A10会产生菊粉酶基因的过表达，以从菊粉中高效产生脂质。

在这项研究中，为了将菊粉直接有效地转化为单细胞油（SCO），将来自马克斯克鲁维酵母的菊粉酶的INU1基因整合到基因组DNA中，并在SCO生产者产黑色素单胞菌P10中积极表达。获得的转化体API41产生28.5U / mL的菊粉酶，并且其野生型菌株P10仅产生8.62U / mL。转化子API41产生的大多数菊粉酶（97.5％）被分泌到培养物中。在10 L发酵过程中，在120小时内从80.0 g / L的菊粉获得了转化子API41酵母细胞中66.2％（w / w）的脂质和14.38 g / L的细胞干重，菊粉酶活性很高（23.7在72小时内也产生了U / mL），并且添加的菊粉被积极水解。这证实了A的基因工程酵母。黑色素原P10适用于直接从菊粉生产脂质。产生的脂质可以用作生物柴油生产的原料。