Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5'-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgFts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5'-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.

中文翻译：

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yqgF突变体中核糖体的亚基组成在大肠杆菌的16S rRNA前加工中不足。

大肠杆菌16S，23S和5S核糖体RNA（rRNA）被转录为单个初级转录本，随后被多个RNase加工成成熟的rRNA。据报道，三种RNA酶（RNase III，RNase E和RNase G）在加工前体16S rRNA（16S rRNA前体）的5'-leader中起作用。以前，我们表明该处理过程涉及一种新颖的必需YqgF。在这里，我们通过LC-MS / MS研究了yqgFts突变体的核糖体亚基。突变核糖体的核糖体蛋白S1的拷贝数减少，表明yqgF基因能够通过处理16S前rRNA的5'端将核糖体蛋白S1掺入核糖体中。核糖体蛋白S1对于在大肠杆菌中翻译至关重要。因此，