While growing on propane as a sole source of carbon, many strains cometabolically degrade environmental pollutants, such as ethers and chlorinated hydrocarbons. To gain insights into the molecular basis behind such a high metabolic versatility of propanotrophs, we examined the propane-inducible protein expression patterns of 2 soil actinobacteria that are known to degrade a variety of ethers (i.e., Mycobacterium sp. strain ENV421 and Rhodococcus sp. strain ENV425). In both strains, soluble diiron monooxygenase(s), that would catalyze the first step of the pathway, were induced by propane. However, despite their phylogenetic similarity, different sets of additional putative propane oxygenases (e.g., cytochrome P450 and particulate methane monooxygenases) were overexpressed in the 2 strains. They also diverged in the expression of enzymes responsible for downstream reactions. This study revealed a diversity of expression of putative propane oxygenases, which may be responsible for xenobiotic degradation, as well as a variety of metabolic pathways for propane in these bacterial species.

中文翻译：

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分枝杆菌中丙烷代谢的比较蛋白质组学分析。菌株ENV421和红球菌sp。菌株ENV425。

当以丙烷作为唯一碳源生长时，许多菌株会通过代谢代谢方式降解环境污染物，例如乙醚和氯化碳氢化合物。为了深入了解原养生物具有如此高的代谢通用性的分子基础，我们研究了两种土壤放线菌的丙烷诱导蛋白质表达模式，已知它们会降解多种醚（例如，分枝杆菌属ENV421菌株和红球菌属。 ENV425）。在两种菌株中，丙烷均诱导了可催化途径第一步的可溶性二铁单加氧酶。然而，尽管它们在系统发育上相似，但是在这两个菌株中过表达了不同的其他假定的丙烷加氧酶（例如，细胞色素P450和颗粒甲烷单加氧酶）集。他们在负责下游反应的酶表达上也有分歧。这项研究揭示了可能的丙烷氧化酶表达的多样性，这可能导致异种生物降解，以及这些细菌物种中丙烷的各种代谢途径。