BackgroundRice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to manage the pest. And stronger virulence will probably emergence when continuous use of insecticides. Therefore, more resistance genes with different resistance mechanisms were needed to be detected and then applied in the rice breeding practice.ResultsResistance genes in the rice variety IR64 were evaluated considering the seedling bulk test and seedling survival rate. As a result, a locus with a large LOD score of 7.23 was found between markers RM302 and YM35 on chromosome 1. The locus explained 36.9% of phenotypic variation and was tentatively denominated Bph37. Moreover, Bph1 was detected to be harbored by the markers RM28366 and RM463, and had the largest LOD score of 2.08, explaining 7.7% of phenotypic variance in the same mapping population. Finally, the preliminary-near-isogenic-lines (pre-NILs) carrying Bph37 exhibited significant tolerance to the insects. But no antibiotic or antixenotic effects were observed in the resistant plants when infested with the insects.ConclusionsWe mapped one major BPH resistance gene Bph37 in consideration of seedling survival rate and the resistance lines showed tolerance to BPH. The detected gene should be beneficial for understanding the resistance mechanism of rice to BPH and for insect-resistance rice breeding programs.

中文翻译：

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水稻品种 IR64 抗褐飞虱数量性状位点的定位和表征

背景稻飞虱（主要褐飞虱，Nilaparvata lugens Stål；BPH）是亚洲水稻生产的主要威胁之一，因为它在田间条件下严重破坏和难以控制。值得注意的是，寄主植物抗性被证明是控制害虫的有效方法之一。并且持续使用杀虫剂可能会出现更强的毒力。因此，需要检测更多具有不同抗性机制的抗性基因，并将其应用于水稻育种实践中。结果水稻品种IR64的抗性基因评价综合考虑了秧苗成活率和成活率。结果，在 1 号染色体上的标记 RM302 和 YM35 之间发现了一个 LOD 得分为 7.23 的位点。该位点解释了 36。9% 的表型变异，暂定名为 Bph37。此外，检测到 Bph1 包含标记 RM28366 和 RM463，并具有 2.08 的最大 LOD 分数，解释了相同作图群体中 7.7% 的表型变异。最后，携带 Bph37 的初步近等基因系 (pre-NIL) 对昆虫表现出显着的耐受性。但在抗虫侵染后，抗性植株未观察到抗生素或抗异种作用。结论考虑到幼苗存活率，我们定位了一个主要的BPH抗性基因Bph37，抗性品系对BPH具有耐受性。检测到的基因有助于了解水稻对BPH的抗性机制和水稻抗虫育种计划。最大的 LOD 得分为 2.08，解释了同一作图群体中 7.7% 的表型变异。最后，携带 Bph37 的初步近等基因系 (pre-NIL) 对昆虫表现出显着的耐受性。但在抗虫侵染后，抗性植株未观察到抗生素或抗异种作用。结论考虑到幼苗存活率，我们定位了一个主要的BPH抗性基因Bph37，抗性品系对BPH具有耐受性。检测到的基因有助于了解水稻对BPH的抗性机制和水稻抗虫育种计划。最大的 LOD 得分为 2.08，解释了同一作图群体中 7.7% 的表型变异。最后，携带 Bph37 的初步近等基因系 (pre-NIL) 对昆虫表现出显着的耐受性。但在抗虫侵染后，抗性植株未观察到抗生素或抗异种作用。结论考虑到幼苗存活率，我们定位了一个主要的BPH抗性基因Bph37，抗性品系对BPH具有耐受性。检测到的基因有助于了解水稻对BPH的抗性机制和水稻抗虫育种计划。携带 Bph37 的初步近等基因系 (pre-NIL) 对昆虫表现出显着的耐受性。但在抗虫侵染后，抗性植株未观察到抗生素或抗异种作用。结论考虑到幼苗存活率，我们定位了一个主要的BPH抗性基因Bph37，抗性品系对BPH具有耐受性。检测到的基因有助于了解水稻对BPH的抗性机制和水稻抗虫育种计划。携带 Bph37 的初步近等基因系 (pre-NIL) 对昆虫表现出显着的耐受性。但在抗虫侵染后，抗性植株未观察到抗生素或抗异种作用。结论考虑到幼苗存活率，我们定位了一个主要的BPH抗性基因Bph37，抗性品系对BPH具有耐受性。检测到的基因有助于了解水稻对BPH的抗性机制和水稻抗虫育种计划。