In this study, we describe the female reproductive system organization and oogenesis in the eutardigrade Thulinius ruffoi. Light, confocal and electron microscopy was used in this study. During oogenesis, three phases can be distinguished: previtellogenesis, vitellogenesis, and choriogenesis. Germ-line cells form cell clusters in which the cells are connected by intercellular (cytoplasmic) bridges. These structures are crucial for delivering the yolk materials, macromolecules, ribosomes, and organelles to the developing oocyte. Vitellogenesis is of a mixed type. Autosynthesis and heterosynthesis of the yolk material occur. Yolk precursors that have been synthesized outside the ovary are delivered to the oocyte via endocytosis. We also present data on cortical granules, and moreover, we describe the cortical reaction in tardigrades, possibly for the first time.

中文翻译：

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Thuffius ruffoi（Tardigrada，Eutardigrada，Isohypsibiidae）的雌性生殖系统和卵子发生。

在这项研究中，我们描述了在欧洲原住民Thulinius ruffoi中的女性生殖系统的组织和卵子发生。在这项研究中使用了光，共聚焦和电子显微镜。在卵子发生过程中，可以区分三个阶段：玻璃体形成，卵黄形成和绒毛膜形成。生殖系细胞形成细胞簇，其中细胞通过细胞间（胞质）桥连接。这些结构对于将卵黄物质，大分子，核糖体和细胞器传递到发育中的卵母细胞至关重要。卵黄发生是混合型。发生蛋黄材料的自合成和杂合成。卵巢外部合成的卵黄前体通过胞吞作用传递到卵母细胞。我们还提供了皮质颗粒的数据，此外，我们还描述了缓坡中的皮质反应，