The purpose of this work was to study changes in the level of cell-free DNA (cfDNA) in the blood of young and old rats in the normal state and with induced benign prostatic hyperplasia (BPH). Male Wistar rats were divided into 4 groups—young (3 months), old (20 months), intact, or with testosterone-induced BPH. Groups with BPH were subjected to surgical castration and administration of testosterone esters at a dose of 25 mg/kg for a total of 7 injections for 20 days. In intact animals, the level of cfDNA in old rats ( ng/μl) was significantly higher than that in the young ( ng/μl). The body and the prostate weights of old rats were 1.6 and 1.4 times larger than those of the young, without an increase in the prostate index (PI). The testosterone level in the blood of young rats was 1.6 times higher than that of old ( nmol/l vs. nmol/l; NS). In animals with BPH, the level of cfDNA in old rats ( ng/μl) was significantly higher than that in young rats ( ng/μl). The body and the prostate weights in old rats were 1.8 and 2.3 times larger, than those in young rats, with an increase in the PI. The level of testosterone in the blood of young ( nmol/l) and old ( nmol/l) rats was not significantly different. Morphological signs of BPH were observed in the prostate of both young and old rats. During the induction of BPH in the experiment, according to the level of cfDNA, cell death processes have not changed significantly in young rats but significantly increased in old rats. A similar trend was observed in the group of intact animals. The obtained data indicate that apoptosis processes are enhanced during the development of BPH despite the growth of tissues in the prostate itself.

中文翻译：

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健康大鼠和去势者睾丸激素诱导的良性前列腺增生的无特定年龄的模式中，无细胞DNA血浆水平不同。

这项工作的目的是研究正常和诱发良性前列腺增生（BPH）的年轻和成年大鼠血液中无细胞DNA（cfDNA）水平的变化。将Wistar雄性大鼠分为4组-年轻（3个月），大（20个月），完整或睾丸激素诱导的BPH。对BPH组进行手术cast割，并以25 mg / kg的剂量给予睾丸激素酯，共7次注射，共20天。在完整的动物中，老年大鼠的cfDNA水平（纳克/ μ升）中的溶液在年轻比显著更高（纳克/ μ 1）。老年大鼠的体重和前列腺重量分别是幼鼠的1.6和1.4倍，而前列腺指数（PI）却没有增加。幼鼠血液中的睾丸激素水平是老鼠的1.6倍（ nmol / l与 nmol / l；NS）。在患有BPH的动物中，老年大鼠中cfDNA的水平（纳克/ μ升）明显高于在年轻大鼠显著更高（纳克/ μ 1）。老年大鼠的身体和前列腺重量分别是幼鼠的1.8和2.3倍，而PI则增加。年轻人血液中睾丸激素的水平（ nmol / l）和旧的（nmol / l）大鼠无显着差异。在年轻和老年大鼠的前列腺中均观察到BPH的形态学征象。在实验中诱导BPH的过程中，根据cfDNA的水平，幼鼠的细胞死亡过程没有明显改变，而老年鼠则明显增加。在完整动物组中观察到了类似的趋势。获得的数据表明，尽管前列腺本身组织生长，但在BPH发生过程中细胞凋亡过程得以增强。