Chemiluminescence (CL) and bioluminescence (BL) imaging technologies, which require no external light source so as to avoid the photobleaching, background interference and autoluminescence, have become powerful tools in biochemical analysis and biomedical science with the development of advanced imaging equipment. CL imaging technology has been widely applied to high-throughput detection of a variety of analytes because of its high sensitivity, high efficiency and high signal-to-noise ratio (SNR). Using luciferase and fluorescent proteins as reporters, various BL imaging systems have been developed innovatively for real-time monitoring of diverse molecules in vivo based on the reaction between luciferin and the substrate. Meanwhile, the kinetics of protein interactions even in deep tissues has been studied by BL imaging. In this review, we summarize in vitro and in vivo applications of CL and BL imaging for biosensing and therapy. We first focus on in vitro CL imaging from the view of improving the sensitivity. Then, in vivo CL applications in cells and tissues based on different CL systems are demonstrated. Subsequently, the recent in vitro and in vivo applications of BL imaging are summarized. Finally, we provide the insight into the development trends and future perspectives of CL and BL imaging technologies.

中文翻译：

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用于生物传感和治疗的化学发光和生物发光成像：体外和体内角度。

化学发光（CL）和生物发光（BL）成像技术不需要外部光源，从而避免了光致漂白，背景干扰和自发光，它们已经成为生物化学分析和生物医学科学中强大的工具，随着先进成像设备的发展。CL成像技术因其高灵敏度，高效率和高信噪比（SNR）而被广泛应用于各种分析物的高通量检测。使用萤光素酶和荧光蛋白作为报告基因，基于萤光素和底物之间的反应，已开发出多种BL成像系统，用于在体内实时监测多种分子。同时，已经通过BL成像研究了甚至在深部组织中蛋白质相互作用的动力学。在这篇评论中 我们总结了CL和BL成像在生物传感和治疗方面的体外和体内应用。从提高灵敏度的角度出发，我们首先关注体外CL成像。然后，展示了基于不同CL系统的体​​内CL在细胞和组织中的应用。随后，总结了BL成像的近期体外和体内应用。最后，我们提供有关CL和BL成像技术的发展趋势和未来前景的见解。