ABSTRACT In recent years, using electromagnetic fields as a targeted therapy for tumors has become a new idea. This paper aims to study the response of rat glioma cells (C6) when the external electromagnetic field parameters change and to obtain a complete working range of magnetic field parameters. Four-day, 4-h daily millisecond magnetic field exposure experiments were performed with C6 cells. The peak values of magnetic field intensity were 260 mT, 90 mT, 19 mT and 6 mT. Each day after exposure, cell morphology and cell viability assay (MTT method) were measured. The response of C6 cells shows a significant window effect and time cumulative effect on the cell, and it is non-destructive. The working inhibited magnetic field range of magnetic field increase rate dB/dt (T/s) is [34, 119.5] and [166.75, 527.25], the magnetic field amplitude B (mT) is [6, 260], the magnetic field integral Bt (mT·s) is [0.1649, 0.8085] and the energy integral B2t (mT2·s) is [2.317, 53.328]. Our findings provide the theoretical and experimental basis for clinical applications of electromagnetic fields.

中文翻译：

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脉冲毫秒电流磁场对C6大鼠胶质瘤细胞增殖的影响

摘要 近年来，利用电磁场作为肿瘤靶向治疗已成为一种新思路。本文旨在研究大鼠神经胶质瘤细胞（C6）在外界电磁场参数变化时的反应，获得完整的磁场参数工作范围。用 C6 细胞进行为期四天、每天 4 小时的毫秒级磁场暴露实验。磁场强度的峰值为260 mT、90 mT、19 mT和6 mT。暴露后的每一天，测量细胞形态和细胞活力测定（MTT 方法）。C6细胞的反应对细胞表现出显着的窗口效应和时间累积效应，并且是非破坏性的。磁场增加率dB/dt（T/s）的工作抑制磁场范围为[34, 119.5]和[166.75, 527.25]，磁场幅度 B (mT) 为 [6, 260]，磁场积分 Bt (mT·s) 为 [0.1649, 0.8085]，能量积分 B2t (mT2·s) 为 [2.317, 53.328]。我们的研究结果为电磁场的临床应用提供了理论和实验基础。