The phylogenetic diversity and antimicrobial resistance (AMR) of Campylobacter coli from humans and animals in Japan between 2008 and 2014 were investigated. A total of 338 foodborne campylobacterioses were reported in Osaka, and C. coli was isolated from 38 cases (11.2%). In the present study, 119 C. coli strains (42 from humans, 25 each from poultry, cattle, and swine, and 2 from wild mallard) were examined by multilocus sequence typing (MLST). MLST assigned 36 sequence types (STs), including 14 novel STs; all human strains and 91% of animal strains (70/77) were assigned to the ST-828 clonal complex. The predominant human ST was ST-860 (18/42, 43%), followed by ST-1068 (8/42, 19%); these STs were also predominant in poultry (ST-860, 9/25, 36%) and cattle (ST-1068, 18/25, 72%). ST-1562 was only predominant in swine (11/25, 44.0%). Swine strains showed the greatest resistance to erythromycin (EM; 92.0%), while EM resistance was only found in 2 out of the 42 human strains examined (4.8%). All EM-resistant swine strains (n=15) exhibited a common point mutation in the 23S rRNA sequence (A2085G), and the tetO gene was detected in 22 out of the 23 TET-resistant swine strains. A whole genome sequencing analysis of four representative swine ST-1562 strains revealed abundant AMR-associated gene clusters in their genomes, suggesting horizontal gene transfer events during host adaptation. This is the first study to demonstrate the phylogenetic diversity and AMR profiles of C. coli in Japan. The present results suggest that poultry and cattle are major reservoirs, improving our knowledge on the epidemiological and ecological traits of this pathogen.

中文翻译：

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日本人和动物弯曲杆菌的系统发育多样性和抗菌素耐药性

研究了 2008 年至 2014 年日本人和动物弯曲杆菌的系统发育多样性和抗菌素耐药性 (AMR)。大阪市共报告食源性弯曲杆菌病 338 例，从 38 例（11.2%）中分离出大肠杆菌。在本研究中，通过多位点序列分型 (MLST) 检查了 119 株大肠杆菌（人类 42 株，家禽、牛和猪各 25 株，野生野鸭 2 株）。MLST 分配了 36 个序列类型 (ST)，其中包括 14 个新的 ST；所有人类品系和 91% 的动物品系 (70/77) 都属于 ST-828 克隆复合体。主要的人类 ST 是 ST-860（18/42，43%），其次是 ST-1068（8/42，19%）；这些 ST 还在家禽 (ST-860, 9/25, 36%) 和牛 (ST-1068, 18/25, 72%) 中占主导地位。ST-1562 仅在猪中占主导地位 (11/25, 44.0%)。猪株对红霉素的抗性最强（EM；92.0%），而 EM 抗性仅在所检查的 42 种人类菌株中的 2 种中发现（4.8%）。所有 EM 抗性猪株（n=15）在 23S rRNA 序列（A2085G）中表现出共同点突变，并且在 23 个 TET 抗性猪株中的 22 个中检测到 tetO 基因。对四种代表性猪 ST-1562 菌株的全基因组测序分析显示，其基因组中有丰富的 AMR 相关基因簇，表明宿主适应期间发生了水平基因转移事件。这是第一项证明日本大肠杆菌系统发育多样性和 AMR 谱的研究。目前的结果表明家禽和牛是主要的宿主，提高了我们对这种病原体的流行病学和生态特征的了解。