JmjC domain containing protein 14 (JMJ14) is an H3K4-specific histone demethylase that plays important roles in RNA-mediated gene silencing and flowering time regulation in Arabidopsis. However, how JMJ14 is recruited to its target genes remains unclear. Here, we show that the C-terminal FYRN and FYRC domains of JMJ14 are required for RNA silencing and flowering time regulation. Chromatin binding of JMJ14 is lost upon deletion of its FYRN and FYRC domains, and H3K4me3 is increased. FYRN and FYRC domains interact with a pair of NAC domain containing transcription factors, NAC050 and NAC052. Genome-wide ChIP analysis revealed that JMJ14 and NAC050/052 share a set of common target genes with CTTGNNNNNCAAG consensus sequences. Mutations in either NAC052 or NAC050 impair RNA-mediated gene silencing. Together, our findings demonstrate an important role of FYRN and FYRC domains in targeting JMJ14 through direct interaction with NAC050/052 proteins, which reveals a novel mechanism of histone demethylase recruitment.

中文翻译：

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组蛋白脱甲基酶的C末端结构域与一对转录因子相互作用，并介导特定的染色质缔合。

包含JmjC域的蛋白质14（JMJ14）是H3K4特异性组蛋白脱甲基酶，在拟南芥中RNA介导的基因沉默和开花时间调控中起重要作用。但是，如何将JMJ14募集到其靶基因仍不清楚。在这里，我们显示JMJ14的C端FYRN和FYRC域对于RNA沉默和开花时间调节是必需的。JMJ14的染色质结合会在其FYRN和FYRC结构域删除后丢失，并且H3K4me3会增加。FYRN和FYRC域与一对包含转录因子NAC050和NAC052的NAC域相互作用。全基因组ChIP分析显示，JMJ14和NAC050 / 052与CTTGNNNNNCAAG共有序列共享一组共同的靶基因。NAC052或NAC050中的突变会削弱RNA介导的基因沉默。一起，