We studied the role of Sirtuin 3 (SIRT3) in microglial cell migration in ischemic stroke. We used a middle cerebral artery occlusion (MCAO) model of focal ischemia. We then applied lentivirus-packaged SIRT3 overexpression and knock down in microglial N9 cells to investigate the underlying mechanism driving microglial cell migration. More microglial cells appeared in the ischemic lesion side after MCAO. The levels of SIRT3 were increased in macrophages, the main source of microglia, after ischemia. CX3CR1 levels were increased with SIRT3 overexpression. SIRT3 promoted microglial N9 cells migration by upregulating CX3CR1 in both normal and glucose deprived culture media. These effects were G protein-dependent. Our study for the first time shows that SIRT3 promotes microglia migration by upregulating CX3CR1.

中文翻译：

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Sirtuin 3通过上调CX3CR1促进小胶质细胞迁移。

我们研究了Sirtuin 3（SIRT3）在缺血性中风中小胶质细胞迁移中的作用。我们使用局灶性脑缺血的大脑中动脉闭塞（MCAO）模型。然后，我们应用慢病毒包装的SIRT3过表达并在小胶质细胞N9细胞中敲低，以研究驱动小胶质细胞迁移的潜在机制。MCAO后缺血性病变侧出现更多的小胶质细胞。缺血后，巨噬细胞（小胶质细胞的主要来源）中SIRT3的水平升高。SIRT3过表达会增加CX3CR1水平。SIRT3通过在正常和葡萄糖缺乏培养基中上调CX3CR1来促进小胶质N9细胞迁移。这些作用是G蛋白依赖性的。我们的首次研究表明，SIRT3通过上调CX3CR1促进小胶质细胞迁移。